Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

• Published in: Macromolecular Rapid Communications
• Main Authors: Miletic, Nemanja, Abetz, Volker, Ebert, Katrin, Loos, Katja
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2010
• Subjects: Candida antarctica lipase B; Enzymes; Nanoparticles; Polystyrene; Antarc*; Antarctica
• Online Access: https://scidar.kg.ac.rs/handle/123456789/19849; https://doi.org/10.1002/marc.200900497

Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. Published