Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobi...

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Bibliographic Details
Published in:Macromolecular Rapid Communications
Main Authors: Miletic, Nemanja, Abetz, Volker, Ebert, Katrin, Loos, Katja
Format: Article in Journal/Newspaper
Language:English
Published: 2010
Subjects:
Candida antarctica lipase B
Enzymes
Nanoparticles
Polystyrene
Antarc*
Antarctica
Online Access:https://scidar.kg.ac.rs/handle/123456789/19849
https://doi.org/10.1002/marc.200900497
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Summary:Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. Published

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