'Gordonibacillus kamchatkensis' gen. nov., sp. nov., a novel member of 'Paenibacillaceae' from frozen volcanic ash

A novel genus and species, Gordonibacillus kamchatkensis gen. nov., sp. nov., with the type strain V-9T (=VKM B-2647T) is proposed to accommodate endospore-forming, rod-shaped and nonmotile bacterium isolated from the frozen volcanic ash sample, Kamchatka Peninsula, Russia. The cell wall of strain V...

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Bibliographic Details
Main Authors: Kudryashova, Ekaterina, Ariskina, Elena, Karlyshev, Andrey, Vinokurova, Natalia, Streshinskaya, Galina, Evtushenko, Lyudmila
Format: Conference Object
Language:unknown
Published: 2018
Subjects:
Online Access:https://eprints.kingston.ac.uk/id/eprint/41952/
Description
Summary:A novel genus and species, Gordonibacillus kamchatkensis gen. nov., sp. nov., with the type strain V-9T (=VKM B-2647T) is proposed to accommodate endospore-forming, rod-shaped and nonmotile bacterium isolated from the frozen volcanic ash sample, Kamchatka Peninsula, Russia. The cell wall of strain V-9T contained a lysine-based peptidoglycan as well as mannose, glucose and glycerol. In addition, two teichoic acids were revealed in the cell wall. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, acylphosphatidylglycerol, and phosphatidylinositol as major components. The fatty acid profile comprised mainly saturated anteiso- and iso-branched acids, with anteiso-C15 predominating (about 50%). The major isoprenologue was MK-7. 50 The genome size of strain V-9T was of 6,957,55 bp., with an average G+C content of 55 mol%. The pairwise average nucleotide identity (ANI) [1] between V-9T and Paenibacillus strains with available genomes calculated using IMG/M (https://img.jgi.doe.gov/cgi-bin/m/main. cgi?section=ANI&page=pairwise) ranged from 69.2 to 73.7%, with 69.7% determined between the target strain and Paenibacillus polymyxa ATCC 842T , the type strain of the type species of the genus. For comparison, the ANI values of 72.85–72.88% were found between V-9T and Thermobacillus composti, Fontibacillus phaseoli and Cohnella thermotolerans, where the latter is the type species of Cohnella. The 16S rRNA gene (1537 bp) pairwise similarity calculated using the EzBioCloud server (http://www.ezbiocloud.net/eztaxon; [2]) showed that strain V-9T had the highest similarity to P. chartarius [3] (96.5%) and P. aestuarii [4] (94.5%). The similarity values between V-9T and type strains of the remaining Paenibacillus species, including the type species of the genus, P. polymyxa [5], did not exceed 91.5% that is below 94.5–95%, a threshold indicative of distinct genera [6, 7]. Most species within the genus Paenibacillus also show low pairwise 16S rRNA gene similarity (<92%), which is evidence of the genus ...