An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas

We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method...

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Bibliographic Details
Main Authors: Zhang, Guofan, Wang, Xiaotong, Song, Xiaorui, Li, Li
Language:unknown
Subjects:
mollusc
bivalve
Pacific oyster
shell DNA
marker-assisted selection (MAS)
Fish culture > Israel.
Fish culture.
Pacific
Crassostrea gigas
Online Access:http://hdl.handle.net/10524/31832
Description
Summary:We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation.

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