Study of Flavobacterium strains isolated from Antarctic aquatic and terrestrial samples
The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore bacterial distribution patterns in Antarctica, nine samples, both terrestrial and aquatic, from different regions were investigated. Isolations were made using several conditions and st...
| Main Authors: | , , , , |
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| Format: | Conference Object |
| Language: | English |
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Society for General Microbiology (SGM)
2009
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| Subjects: | |
| Online Access: | https://biblio.ugent.be/publication/936632 http://hdl.handle.net/1854/LU-936632 |
| Summary: | The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore bacterial distribution patterns in Antarctica, nine samples, both terrestrial and aquatic, from different regions were investigated. Isolations were made using several conditions and strains were then subjected to rep-PCR fingerprinting as a fast screening to eliminate duplicate isolates. Cluster analysis of fingerprint patterns using BioNumerics software revealed a number of clusters (cut-off level 80%) of similar strains and a number of separate isolates. Representatives were used in partial 16S rDNA sequencing to obtain a first approximate identification. The results show a large diversity, distributed over the major phylogenetic groups and only little overlap between the samples. We focussed on the genus Flavobacterium because several of the isolated clusters and strains that were found in this genus show low similarity values with neighbouring sequences in the EMBL-database and thus may represent new species. The 16S rDNA sequence was completed and fatty acid analysis as well as some phenotypic tests were performed. A housekeeping gene, gyrB, is being sequenced and, if required DNA-DNA hybridizations will be carried out, to establish whether these isolates can be described as new species. |
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