Development of a culture protocol for Rhodomonas sp. Hf-1 strain through laboratory trials

Rhodomonas sp. Hf-1 is a marine microalgal strain with a high nutritional value. In order to produce this microalga effectively and inexpensively in a hatchery, its production protocol was reexamined through several culture trials. Firstly, it was found that the Hf-1 strain required nitrogen, phosph...

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Bibliographic Details
Published in:Fisheries Science
Main Authors: Yamamoto, Satoshi, Yamato, Reina, Aritaki, Yudai, Bossier, Peter, Yoshimatsu, Takao
Format: Article in Journal/Newspaper
Language:English
Published: 2019
Subjects:
Agriculture and Food Sciences
Biology and Life Sciences
Essential nutrients
Optimal nitrogen source
Semi-continuous culture system
Marine microalga
FATTY-ACID-COMPOSITION
TOTAL LIPID-CONTENT
BIOMASS PRODUCTION
BIOCHEMICAL-COMPOSITION
CHLORELLA-SOROKINIANA
TROPICAL AQUACULTURE
CRASSOSTREA-GIGAS
LIVE FOOD
MICROALGAE
GROWTH
Crassostrea gigas
Online Access:https://biblio.ugent.be/publication/8650754
http://hdl.handle.net/1854/LU-8650754
https://doi.org/10.1007/s12562-019-01325-z
https://biblio.ugent.be/publication/8650754/file/8650755
Description
Summary:Rhodomonas sp. Hf-1 is a marine microalgal strain with a high nutritional value. In order to produce this microalga effectively and inexpensively in a hatchery, its production protocol was reexamined through several culture trials. Firstly, it was found that the Hf-1 strain required nitrogen, phosphorus, iron, manganese and vitamin B-12. The optimal nitrogen source was urea. Based on these results, a new medium, MU-SW, was formulated from agricultural fertilizers. Secondly, MU-SW medium was verified at production scale, in which satisfactory growth of the Hf-1 strain was obtained. The cell density reached approximately 3.0x10(6)cellsml(-1), and five harvest cycles at 4-day intervals could be achieved. The high unsaturated fatty acid content of the harvested cells fluctuated in the following ranges: C18:2n-6, 19.8-25.2%; C18:3n-6, 8.7-15.2%; C20:4n-6, 2.0-4.6%; C20:5n-3, 8.2-11.3% and C22:6n-3, 5.6-7.3%. This study provides the baseline conditions for introducing the Hf-1 strain into an aquaculture food chain.

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