Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus
Poly- and monoclonal antibodies, raised against mammalian membrane-bound proton Dump (V-ATPase) were applied to the bone-resorbing cells of Oreochromis niloticus to clarify if osteoclasts of an advanced teleost species display V-ATPase, a key enzyme in the process of bone resorption. All antibodies...
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ftunivgent:oai:archive.ugent.be:8646067 2023-06-11T04:03:45+02:00 Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus Witten, Paul Eckhard Holliday, L. S. Delling, G. Hall, B. K. 1999 application/pdf https://biblio.ugent.be/publication/8646067 http://hdl.handle.net/1854/LU-8646067 https://doi.org/10.1111/j.1095-8649.1999.tb02074.x https://biblio.ugent.be/publication/8646067/file/8646068 eng eng https://biblio.ugent.be/publication/8646067 http://hdl.handle.net/1854/LU-8646067 http://dx.doi.org/10.1111/j.1095-8649.1999.tb02074.x https://biblio.ugent.be/publication/8646067/file/8646068 No license (in copyright) info:eu-repo/semantics/restrictedAccess JOURNAL OF FISH BIOLOGY ISSN: 0022-1112 ISSN: 1095-8649 Biology and Life Sciences Aquatic Science Ecology Evolution Behavior and Systematics proton pump V-ATPase osteoclasts acellular bone TRAP skeletal development RESISTANT ACID-PHOSPHATASE ANGUILLA-ANGUILLA-L TARTRATE-RESISTANT IN-VITRO ONCORHYNCHUS-MYKISS HUMAN OSTEOCLAST RAINBOW-TROUT CICHLID FISH MOUSE BONE journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1999 ftunivgent https://doi.org/10.1111/j.1095-8649.1999.tb02074.x 2023-05-10T22:38:27Z Poly- and monoclonal antibodies, raised against mammalian membrane-bound proton Dump (V-ATPase) were applied to the bone-resorbing cells of Oreochromis niloticus to clarify if osteoclasts of an advanced teleost species display V-ATPase, a key enzyme in the process of bone resorption. All antibodies labelled cells at known sites of bone resorption, the endosteal bone surfaces surrounding the tooth anlagen. The best results were achieved with a monoclonal antibody (E11). Although the majority of labelled cells were flat and mononucleated, the occurrence of V-ATPase in these cells indicates that they function as active bone-resorbing cells. The monoclonal antibody E11 was also applied successfully to monocytes, cells that are believed to be related most closely to osteoclasts. The assignment of V-ATPase to bone resorbing cells of O. niloticus was confirmed by application of the additional osteoclast markers, tartrate-resistant acid phosphatase (TRAP) and tartrate-resistant ATPase (TraATPase). Co-expression of V-ATPase, TRAP and TraATPase in fish osteoclasts is demonstrated for the first time. (C) 1999 The Fisheries Society of the British Isles. Article in Journal/Newspaper Anguilla anguilla Ghent University Academic Bibliography Journal of Fish Biology 55 6 1258 1272 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Aquatic Science Ecology Evolution Behavior and Systematics proton pump V-ATPase osteoclasts acellular bone TRAP skeletal development RESISTANT ACID-PHOSPHATASE ANGUILLA-ANGUILLA-L TARTRATE-RESISTANT IN-VITRO ONCORHYNCHUS-MYKISS HUMAN OSTEOCLAST RAINBOW-TROUT CICHLID FISH MOUSE BONE |
spellingShingle |
Biology and Life Sciences Aquatic Science Ecology Evolution Behavior and Systematics proton pump V-ATPase osteoclasts acellular bone TRAP skeletal development RESISTANT ACID-PHOSPHATASE ANGUILLA-ANGUILLA-L TARTRATE-RESISTANT IN-VITRO ONCORHYNCHUS-MYKISS HUMAN OSTEOCLAST RAINBOW-TROUT CICHLID FISH MOUSE BONE Witten, Paul Eckhard Holliday, L. S. Delling, G. Hall, B. K. Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
topic_facet |
Biology and Life Sciences Aquatic Science Ecology Evolution Behavior and Systematics proton pump V-ATPase osteoclasts acellular bone TRAP skeletal development RESISTANT ACID-PHOSPHATASE ANGUILLA-ANGUILLA-L TARTRATE-RESISTANT IN-VITRO ONCORHYNCHUS-MYKISS HUMAN OSTEOCLAST RAINBOW-TROUT CICHLID FISH MOUSE BONE |
description |
Poly- and monoclonal antibodies, raised against mammalian membrane-bound proton Dump (V-ATPase) were applied to the bone-resorbing cells of Oreochromis niloticus to clarify if osteoclasts of an advanced teleost species display V-ATPase, a key enzyme in the process of bone resorption. All antibodies labelled cells at known sites of bone resorption, the endosteal bone surfaces surrounding the tooth anlagen. The best results were achieved with a monoclonal antibody (E11). Although the majority of labelled cells were flat and mononucleated, the occurrence of V-ATPase in these cells indicates that they function as active bone-resorbing cells. The monoclonal antibody E11 was also applied successfully to monocytes, cells that are believed to be related most closely to osteoclasts. The assignment of V-ATPase to bone resorbing cells of O. niloticus was confirmed by application of the additional osteoclast markers, tartrate-resistant acid phosphatase (TRAP) and tartrate-resistant ATPase (TraATPase). Co-expression of V-ATPase, TRAP and TraATPase in fish osteoclasts is demonstrated for the first time. (C) 1999 The Fisheries Society of the British Isles. |
format |
Article in Journal/Newspaper |
author |
Witten, Paul Eckhard Holliday, L. S. Delling, G. Hall, B. K. |
author_facet |
Witten, Paul Eckhard Holliday, L. S. Delling, G. Hall, B. K. |
author_sort |
Witten, Paul Eckhard |
title |
Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
title_short |
Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
title_full |
Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
title_fullStr |
Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
title_full_unstemmed |
Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus |
title_sort |
immunohistochemical identification of a vacuolar proton pump (v-atpase) in bone-resorbing cells of an advanced teleost species, oreochromis niloticus |
publishDate |
1999 |
url |
https://biblio.ugent.be/publication/8646067 http://hdl.handle.net/1854/LU-8646067 https://doi.org/10.1111/j.1095-8649.1999.tb02074.x https://biblio.ugent.be/publication/8646067/file/8646068 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
JOURNAL OF FISH BIOLOGY ISSN: 0022-1112 ISSN: 1095-8649 |
op_relation |
https://biblio.ugent.be/publication/8646067 http://hdl.handle.net/1854/LU-8646067 http://dx.doi.org/10.1111/j.1095-8649.1999.tb02074.x https://biblio.ugent.be/publication/8646067/file/8646068 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1095-8649.1999.tb02074.x |
container_title |
Journal of Fish Biology |
container_volume |
55 |
container_issue |
6 |
container_start_page |
1258 |
op_container_end_page |
1272 |
_version_ |
1768383016750546944 |