Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone-resorbing cells of an advanced teleost species, Oreochromis niloticus

Poly- and monoclonal antibodies, raised against mammalian membrane-bound proton Dump (V-ATPase) were applied to the bone-resorbing cells of Oreochromis niloticus to clarify if osteoclasts of an advanced teleost species display V-ATPase, a key enzyme in the process of bone resorption. All antibodies...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Witten, Paul Eckhard, Holliday, L. S., Delling, G., Hall, B. K.
Format: Article in Journal/Newspaper
Language:English
Published: 1999
Subjects:
Biology and Life Sciences
Aquatic Science
Ecology
Evolution
Behavior and Systematics
proton pump
V-ATPase
osteoclasts
acellular bone
TRAP
skeletal development
RESISTANT ACID-PHOSPHATASE
ANGUILLA-ANGUILLA-L
TARTRATE-RESISTANT
IN-VITRO
ONCORHYNCHUS-MYKISS
HUMAN OSTEOCLAST
RAINBOW-TROUT
CICHLID FISH
MOUSE BONE
Anguilla anguilla
Online Access:https://biblio.ugent.be/publication/8646067
http://hdl.handle.net/1854/LU-8646067
https://doi.org/10.1111/j.1095-8649.1999.tb02074.x
https://biblio.ugent.be/publication/8646067/file/8646068
Description
Summary:Poly- and monoclonal antibodies, raised against mammalian membrane-bound proton Dump (V-ATPase) were applied to the bone-resorbing cells of Oreochromis niloticus to clarify if osteoclasts of an advanced teleost species display V-ATPase, a key enzyme in the process of bone resorption. All antibodies labelled cells at known sites of bone resorption, the endosteal bone surfaces surrounding the tooth anlagen. The best results were achieved with a monoclonal antibody (E11). Although the majority of labelled cells were flat and mononucleated, the occurrence of V-ATPase in these cells indicates that they function as active bone-resorbing cells. The monoclonal antibody E11 was also applied successfully to monocytes, cells that are believed to be related most closely to osteoclasts. The assignment of V-ATPase to bone resorbing cells of O. niloticus was confirmed by application of the additional osteoclast markers, tartrate-resistant acid phosphatase (TRAP) and tartrate-resistant ATPase (TraATPase). Co-expression of V-ATPase, TRAP and TraATPase in fish osteoclasts is demonstrated for the first time. (C) 1999 The Fisheries Society of the British Isles.

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