Scrutinizing the triad of Vibrio tapetis, the skin barrier and pigmentation as determining factors in the development of skin ulcerations in wild common dab (Limanda limanda)

Recently, Vibrio tapetis was isolated for the first time from skin ulcerations in wild-caught common dab (Limanda limanda). To further examine its role in the development of these skin lesions, an in vivo experiment was performed. The significance of the skin barrier and in addition the difference b...

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Bibliographic Details
Published in:Veterinary Research
Main Authors: Vercauteren, Maaike, De Swaef, Evelien, Declercq, Annelies, Polet, Hans, Aerts, Johan, Ampe, Bart, Romalde, Jesus L., Haesebrouck, Freddy, Devriese, Lisa, Decostere, Annemie, Chiers, Koen
Format: Article in Journal/Newspaper
Language:English
Published: 2019
Subjects:
Veterinary Sciences
FLOUNDER PLATICHTHYS-FLESUS
DUTCH WADDEN SEA
ATLANTIC SALMON
EPIDERMAL PAPILLOMA
SYMPHODUS-MELOPS
CORKWING WRASSE
ULCER DISEASE
STRAINS
STRESS
MUCUS
Atlantic salmon
Online Access:https://biblio.ugent.be/publication/8623880
http://hdl.handle.net/1854/LU-8623880
https://doi.org/10.1186/s13567-019-0659-6
https://biblio.ugent.be/publication/8623880/file/8623881
Description
Summary:Recently, Vibrio tapetis was isolated for the first time from skin ulcerations in wild-caught common dab (Limanda limanda). To further examine its role in the development of these skin lesions, an in vivo experiment was performed. The significance of the skin barrier and in addition the difference between pigmented and non-pigmented side were investigated. Hence, the skin of common dab was treated in three different ways on both the pigmented and non-pigmented side. On a first treatment zone, the scales and overlying epidermal tissue were removed whereas in a second zone only the mucus was discarded. The third zone served as a non-treated zone. Thereafter, fish were challenged with V. tapetis. The control group was sham treated. Mortality, clinical signs, severity and size of the developing lesions were recorded. All animals were sacrificed and sampled 21days post-inoculation. Significantly more fish of the group challenged with V. tapetis died compared to the control group with the highest incidence occurring 4days post-inoculation. Fish challenged with V. tapetis developed more severe skin ulcerations. In zones where scales and epidermal tissue were removed, the ulcerations were more severe compared to zones where only mucus was eliminated. Ulcerations occurred more frequently, were more severe and larger on the pigmented side. Our data represents prove of V. tapetis as causative agent of ulcerative skin lesions although prior damage of the skin seems to be a major contributing factor. Furthermore, the pigmented side seemed predisposed to the development of skin ulcerations.

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