Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature

Psychrobacter arcticus 273-4 is a Gram-negative bacterium isolated from a 20,000-to-30,000-year-old continuously frozen permafrost in the Kolyma region in Siberia. The survival strategies adopted to live at subzero temperatures include all the outer membrane molecules. A strategic involvement in the...

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Bibliographic Details
Published in:Extremophiles
Main Authors: Casillo, Angela, Ziaco, Marcello, Lindner, Buko, Parrilli, Ermenegilda, Schwudke, Dominik, Holgado Muñoz, Aurora, Beyaert, Rudi, Lanzetta, Rosa, Tutino, Maria Luisa, Corsaro, Maria Michela
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
Biology and Life Sciences
PSEUDOALTEROMONAS-HALOPLANKTIS TAC-125
FATTY-ACID DISTRIBUTION
BIOLOGICAL-ACTIVITY
MASS-SPECTROMETRY
PERMAFROST
BACTERIUM
ADAPTATION
LIPOOLIGOSACCHARIDE
CRYOHALOLENTIS
MICROORGANISMS
Psychrobacter arcticus 273-4
Lipid A
Mass spectrometry
Psychrotolerant
Kolyma
Tac
permafrost
Siberia
Online Access:https://biblio.ugent.be/publication/8586411
http://hdl.handle.net/1854/LU-8586411
https://doi.org/10.1007/s00792-018-1051-6
https://biblio.ugent.be/publication/8586411/file/8586412
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Summary:Psychrobacter arcticus 273-4 is a Gram-negative bacterium isolated from a 20,000-to-30,000-year-old continuously frozen permafrost in the Kolyma region in Siberia. The survival strategies adopted to live at subzero temperatures include all the outer membrane molecules. A strategic involvement in the well-known enhancement of cellular membrane fluidity is attributable to the lipopolysaccharides (LPSs). These molecules covering about the 75% of cellular surface contribute to cold adaptation through structural modifications in their portions. In this work, we elucidated the exact structure of lipid A moiety obtained from the lipopolysaccharide of P. arcticus grown at 4 degrees C, to mimic the response to the real environment temperatures. The lipid A was obtained from the LPS by mild acid hydrolysis. The lipid A and its partially deacylated derivatives were exhaustively characterized by chemical analysis and by means of ESI Q-Orbitrap mass spectrometry. Moreover, biological assays indicated that P. arcticus 273-4 lipid A may behave as a weak TLR4 agonist.

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