A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka

osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and...

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Bibliographic Details
Published in:Development
Main Authors: Yu, Tingsheng, Graf, Martin, Renn, Joerg, Schartl, Manfred, Larionova, Daria, Huysseune, Ann, Witten, Paul Eckhard, Winkler, Christoph
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Biology and Life Sciences
SALMON SALMO-SALAR
BONE-FORMATION
OSTEOBLAST DIFFERENTIATION
AXIAL
SKELETON
RETINOIC ACID
COMMON CARP
ZEBRAFISH
CELLS
MINERALIZATION
EVOLUTION
Osteoblasts
Osteogenesis
Bone modelling
Medaka
Salmo salar
Online Access:https://biblio.ugent.be/publication/8525440
http://hdl.handle.net/1854/LU-8525440
https://doi.org/10.1242/dev.139550
https://biblio.ugent.be/publication/8525440/file/8606199
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Summary:osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.

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