Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae
The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusio...
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ftunivgent:oai:archive.ugent.be:527415 2023-06-11T04:11:47+02:00 Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae Dierckens, Kristof Rekecki, Anamaria Laureau, S Sorgeloos, Patrick Boon, Nico Van Den Broeck, Wim Bossier, Peter 2009 application/pdf https://biblio.ugent.be/publication/527415 http://hdl.handle.net/1854/LU-527415 https://doi.org/10.1111/j.1462-2920.2008.01794.x https://biblio.ugent.be/publication/527415/file/537948 eng eng https://biblio.ugent.be/publication/527415 http://hdl.handle.net/1854/LU-527415 http://dx.doi.org/10.1111/j.1462-2920.2008.01794.x https://biblio.ugent.be/publication/527415/file/537948 No license (in copyright) info:eu-repo/semantics/restrictedAccess ENVIRONMENTAL MICROBIOLOGY ISSN: 1462-2912 Biology and Life Sciences GADUS-MORHUA L TURBOT SCOPHTHALMUS-MAXIMUS VIBRIO-ANGUILLARUM ATLANTIC HALIBUT LISTONELLA-ANGUILLARUM MYCOBACTERIUM-MARINUM AEROMONAS-HYDROPHILA NONCULTURABLE STATE ARTEMIA JUVENILES MICROBIAL CONTROL journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2009 ftunivgent https://doi.org/10.1111/j.1462-2920.2008.01794.x 2023-04-19T22:09:35Z The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusions. In this study, a gnotobiotic sea bass larvae (Dicentrarchus labrax) test system was developed. In order to obtain bacteria-free animals, the eggs were disinfected with glutaraldehyde and subsequently incubated in a solution of rifampicin and ampicillin. Axenity was confirmed using culture-dependent and -independent techniques. The gnotobiotic larvae were fed axenic Artemia sp. from 7 days after hatching onwards. In the challenge test, one of the three opportunistic pathogens, Aeromonas hydrophila, Listonella anguillarum serovar O1 and O2a, was added to the model system via the water and encapsulated in Artemia sp. Only serovar O2a led to increased mortality in the sea bass larvae. The presented gnotobiotic model can be used for research on, among others, reciprocal metabolic effects between microorganisms and the host (e.g. as measured by gene expression), immunostimulants, pharmacological research and the histological development of the gastrointestinal tract and growth of larvae. Article in Journal/Newspaper Gadus morhua Scophthalmus maximus Turbot Ghent University Academic Bibliography Environmental Microbiology 11 2 526 533 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences GADUS-MORHUA L TURBOT SCOPHTHALMUS-MAXIMUS VIBRIO-ANGUILLARUM ATLANTIC HALIBUT LISTONELLA-ANGUILLARUM MYCOBACTERIUM-MARINUM AEROMONAS-HYDROPHILA NONCULTURABLE STATE ARTEMIA JUVENILES MICROBIAL CONTROL |
spellingShingle |
Biology and Life Sciences GADUS-MORHUA L TURBOT SCOPHTHALMUS-MAXIMUS VIBRIO-ANGUILLARUM ATLANTIC HALIBUT LISTONELLA-ANGUILLARUM MYCOBACTERIUM-MARINUM AEROMONAS-HYDROPHILA NONCULTURABLE STATE ARTEMIA JUVENILES MICROBIAL CONTROL Dierckens, Kristof Rekecki, Anamaria Laureau, S Sorgeloos, Patrick Boon, Nico Van Den Broeck, Wim Bossier, Peter Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
topic_facet |
Biology and Life Sciences GADUS-MORHUA L TURBOT SCOPHTHALMUS-MAXIMUS VIBRIO-ANGUILLARUM ATLANTIC HALIBUT LISTONELLA-ANGUILLARUM MYCOBACTERIUM-MARINUM AEROMONAS-HYDROPHILA NONCULTURABLE STATE ARTEMIA JUVENILES MICROBIAL CONTROL |
description |
The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusions. In this study, a gnotobiotic sea bass larvae (Dicentrarchus labrax) test system was developed. In order to obtain bacteria-free animals, the eggs were disinfected with glutaraldehyde and subsequently incubated in a solution of rifampicin and ampicillin. Axenity was confirmed using culture-dependent and -independent techniques. The gnotobiotic larvae were fed axenic Artemia sp. from 7 days after hatching onwards. In the challenge test, one of the three opportunistic pathogens, Aeromonas hydrophila, Listonella anguillarum serovar O1 and O2a, was added to the model system via the water and encapsulated in Artemia sp. Only serovar O2a led to increased mortality in the sea bass larvae. The presented gnotobiotic model can be used for research on, among others, reciprocal metabolic effects between microorganisms and the host (e.g. as measured by gene expression), immunostimulants, pharmacological research and the histological development of the gastrointestinal tract and growth of larvae. |
format |
Article in Journal/Newspaper |
author |
Dierckens, Kristof Rekecki, Anamaria Laureau, S Sorgeloos, Patrick Boon, Nico Van Den Broeck, Wim Bossier, Peter |
author_facet |
Dierckens, Kristof Rekecki, Anamaria Laureau, S Sorgeloos, Patrick Boon, Nico Van Den Broeck, Wim Bossier, Peter |
author_sort |
Dierckens, Kristof |
title |
Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
title_short |
Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
title_full |
Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
title_fullStr |
Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
title_full_unstemmed |
Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae |
title_sort |
development of a bacterial challenge test for gnotobiotic sea bass (dicentrarchus labrax) larvae |
publishDate |
2009 |
url |
https://biblio.ugent.be/publication/527415 http://hdl.handle.net/1854/LU-527415 https://doi.org/10.1111/j.1462-2920.2008.01794.x https://biblio.ugent.be/publication/527415/file/537948 |
genre |
Gadus morhua Scophthalmus maximus Turbot |
genre_facet |
Gadus morhua Scophthalmus maximus Turbot |
op_source |
ENVIRONMENTAL MICROBIOLOGY ISSN: 1462-2912 |
op_relation |
https://biblio.ugent.be/publication/527415 http://hdl.handle.net/1854/LU-527415 http://dx.doi.org/10.1111/j.1462-2920.2008.01794.x https://biblio.ugent.be/publication/527415/file/537948 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01794.x |
container_title |
Environmental Microbiology |
container_volume |
11 |
container_issue |
2 |
container_start_page |
526 |
op_container_end_page |
533 |
_version_ |
1768387111602356224 |