Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae

The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusio...

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Bibliographic Details
Published in:Environmental Microbiology
Main Authors: Dierckens, Kristof, Rekecki, Anamaria, Laureau, S, Sorgeloos, Patrick, Boon, Nico, Van Den Broeck, Wim, Bossier, Peter
Format: Article in Journal/Newspaper
Language:English
Published: 2009
Subjects:
Biology and Life Sciences
GADUS-MORHUA L
TURBOT SCOPHTHALMUS-MAXIMUS
VIBRIO-ANGUILLARUM
ATLANTIC HALIBUT
LISTONELLA-ANGUILLARUM
MYCOBACTERIUM-MARINUM
AEROMONAS-HYDROPHILA
NONCULTURABLE STATE
ARTEMIA JUVENILES
MICROBIAL CONTROL
Gadus morhua
Scophthalmus maximus
Turbot
Online Access:https://biblio.ugent.be/publication/527415
http://hdl.handle.net/1854/LU-527415
https://doi.org/10.1111/j.1462-2920.2008.01794.x
https://biblio.ugent.be/publication/527415/file/537948
Description
Summary:The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusions. In this study, a gnotobiotic sea bass larvae (Dicentrarchus labrax) test system was developed. In order to obtain bacteria-free animals, the eggs were disinfected with glutaraldehyde and subsequently incubated in a solution of rifampicin and ampicillin. Axenity was confirmed using culture-dependent and -independent techniques. The gnotobiotic larvae were fed axenic Artemia sp. from 7 days after hatching onwards. In the challenge test, one of the three opportunistic pathogens, Aeromonas hydrophila, Listonella anguillarum serovar O1 and O2a, was added to the model system via the water and encapsulated in Artemia sp. Only serovar O2a led to increased mortality in the sea bass larvae. The presented gnotobiotic model can be used for research on, among others, reciprocal metabolic effects between microorganisms and the host (e.g. as measured by gene expression), immunostimulants, pharmacological research and the histological development of the gastrointestinal tract and growth of larvae.

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