Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots

Infection with ectomycorrhizal fungi can increase the ability of plants to resist drought stress through morphophysiological and biochemical mechanisms. However, the metabolism of antioxidative enzyme activities in the ectomycorrhizal symbiosis remains poorly understood. This study investigated biom...

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Bibliographic Details
Published in:Tree Physiology
Main Authors: Alvarez, Maricel, Huygens, Dries, Fernandez, Carlos, Gacitua, Yessy, Olivares, Erick, Saavedra, Isabel, Alberdi, Miren, Valenzuela, Eduardo
Format: Article in Journal/Newspaper
Language:English
Published: 2009
Subjects:
Biology and Life Sciences
SOYBEAN PLANTS
NITRIC-OXIDE
NODULE SENESCENCE
ANTIOXIDANT SYSTEMS
LYCOPERSICON-PENNELLII
HYDROGEN-PEROXIDE
OXIDATIVE STRESS
WATER-STRESS
ASCORBATE-GLUTATHIONE CYCLE
SUPEROXIDE-DISMUTASE ACTIVITY
volcanic soil
superoxide dismutase
pristine forest
glutathione reductase
ascorbate peroxidase
catalase
antartic*
Online Access:https://biblio.ugent.be/publication/2120390
http://hdl.handle.net/1854/LU-2120390
https://doi.org/10.1093/treephys/tpp038
https://biblio.ugent.be/publication/2120390/file/2120622
Description
Summary:Infection with ectomycorrhizal fungi can increase the ability of plants to resist drought stress through morphophysiological and biochemical mechanisms. However, the metabolism of antioxidative enzyme activities in the ectomycorrhizal symbiosis remains poorly understood. This study investigated biomass production, reactive oxygen metabolism (hydrogen peroxide and malondialdehyde concentration) and antioxidant enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in pure cultures of the ectomycorrhizal fungi Descolea antartica Sing. and Pisolithus tinctorius (Pers.) Coker & Couch, and non-mycorrhizal and mycorrhizal roots of Nothofagus dombeyi (Mirb.) roots under well-watered conditions and drought conditions (DC). The studied ectomycorrhizal fungi regulated their antioxidative enzyme metabolism differentially in response to drought, resulting in cellular damage in D. antartica but not in P. tinctorius. Ectomycorrhizal inoculation and water treatment had a significant effect on all parameters studied, including relative water content of the plant. As such, N. dombeyi plants in symbiosis experienced a lower oxidative stress effect than non-mycorrhizal plants under DC. Additionally, ectomycorrhizal N. dombeyi roots showed a greater antioxidant enzyme activity relative to non-mycorrhizal roots, an effect which was further expressed under DC. The association between the non-specific P. tinctorius and N. dombeyi had a more effective reactive oxygen species (ROS) metabolism than the specific D. antartica-N. dombeyi symbiosis. We conclude that the combination of effective ROS prevention and ROS detoxification by ectomycorrhizal plants resulted in reduced cellular damage and increased plant growth relative to non-mycorrhizal plants under drought.

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