Effect of different light regimes on the docosahexaenoic acid (DHA) content of Isochrysis aff. galbana (clone T-ISO)

The culture of Isochrysis aff. galbana Green (T-ISO) in hatcheries is promoted for its high docosahexaenoic acid (DHA) content which favours the successful rearing of aquatic larvae. In this paper we identified the need for well-defined culture conditions of microalgae if sufficient DHA content is t...

Full description

Bibliographic Details
Main Authors: Tzovenis, I, De Pauw, Niels, Sorgeloos, Patrick
Format: Article in Journal/Newspaper
Language:English
Published: 1997
Subjects:
Biology and Life Sciences
docosahexaenoic acid (DHA)
clonal variation
factorial optimization
light limitation
photoperiod
T-ISO
CLAM TAPES-PHILIPPINARUM
MARINE UNICELLULAR ALGAE
SCOPHTHALMUS-MAXIMUS L
FATTY-ACID
LIPID-COMPOSITION
BIOCHEMICAL-COMPOSITION
TROPICAL AQUACULTURE
L LARVAE
NANNOCHLOROPSIS-OCULATA
CHEMICAL-COMPOSITION
Scophthalmus maximus
Online Access:https://biblio.ugent.be/publication/186102
http://hdl.handle.net/1854/LU-186102
https://doi.org/10.1023/A:1018349131522
https://biblio.ugent.be/publication/186102/file/2108976
Description
Summary:The culture of Isochrysis aff. galbana Green (T-ISO) in hatcheries is promoted for its high docosahexaenoic acid (DHA) content which favours the successful rearing of aquatic larvae. In this paper we identified the need for well-defined culture conditions of microalgae if sufficient DHA content is the final goal. Using photoperiods of 24:0 h, 12:12 h, and 16:8 h L/D combined with photon flux densities (PFDs) of 110, 220, and 460 mu E m(-2) s(-1), batch cultures of T-ISO were sampled for DHA content in the exponential as well as the post-exponential (light limited) growth phase. The various light regimes resulted in differences of DHA content of T-ISO revealing influences and strong interactions among the three variables studied. For fast and economic production of T-ISO cells rich in DHA it is recommended to harvest batch cultures at the end of the exponential phase using high photon flux densities (PFDs) under 12:12 h L/D cycles. The same regime or lower PFD under 24:0 h L/D seem to be sufficient for a continuous culture optimization scheme. In any case a strict following of culture protocols is very important since there is evidence for coexistence of different clones within the parental cultures leading to different results depending on which one prevails.

Similar Items