Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4
Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacteri...
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ftunivgent:oai:archive.ugent.be:1191499 2023-10-01T03:59:19+02:00 Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 Han, Yin Li, X Qi, Zizhong Zhang, XH Bossier, Peter 2010 application/pdf https://biblio.ugent.be/publication/1191499 http://hdl.handle.net/1854/LU-1191499 https://doi.org/10.1111/j.1365-2672.2009.04405.x https://biblio.ugent.be/publication/1191499/file/1191517 eng eng https://biblio.ugent.be/publication/1191499 http://hdl.handle.net/1854/LU-1191499 http://dx.doi.org/10.1111/j.1365-2672.2009.04405.x https://biblio.ugent.be/publication/1191499/file/1191517 No license (in copyright) info:eu-repo/semantics/restrictedAccess JOURNAL OF APPLIED MICROBIOLOGY ISSN: 1364-5072 Agriculture and Food Sciences cholerae autoinducer 1 N-acylhomoserine lactones autoinducer 2 Edwardsiella tarda quorum sensing VIBRIO-HARVEYI PSEUDOMONAS-AERUGINOSA AEROMONAS-HYDROPHILA GENE-EXPRESSION LUXRI HOMOLOGS LUMINESCENCE AUTOINDUCER SYSTEMS IDENTIFICATION INHIBITION journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2010 ftunivgent https://doi.org/10.1111/j.1365-2672.2009.04405.x 2023-09-06T22:24:15Z Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria. LTB-4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer-2 (AI-2). The AI-2 activity produced by LTB-4 was growth phase dependent and topped at OD600 of 1 center dot 0. The protocol to detect cholerae autoinducer 1 (CAI-1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI-1 activity could not be detected in LTB-4. Conclusion: Edwardsiella tarda LTB-4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL-inducing QS, AI-2 may be the first predominant signal, functioning at early exponential phase. LTB-4 did not produce any CAI-1 activity. Significance and Impact of the Study: Different QS signal molecules of Edw. tarda LTB-4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB-4 produced at least four kinds of AHLs, which seemed to be C-4-HSL, C-6-HSL, 3-oxo-C-6-HSL and an uncharacterized AHL molecule. Article in Journal/Newspaper Scophthalmus maximus Turbot Ghent University Academic Bibliography Journal of Applied Microbiology 108 1 139 147 |
institution |
Open Polar |
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Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Agriculture and Food Sciences cholerae autoinducer 1 N-acylhomoserine lactones autoinducer 2 Edwardsiella tarda quorum sensing VIBRIO-HARVEYI PSEUDOMONAS-AERUGINOSA AEROMONAS-HYDROPHILA GENE-EXPRESSION LUXRI HOMOLOGS LUMINESCENCE AUTOINDUCER SYSTEMS IDENTIFICATION INHIBITION |
spellingShingle |
Agriculture and Food Sciences cholerae autoinducer 1 N-acylhomoserine lactones autoinducer 2 Edwardsiella tarda quorum sensing VIBRIO-HARVEYI PSEUDOMONAS-AERUGINOSA AEROMONAS-HYDROPHILA GENE-EXPRESSION LUXRI HOMOLOGS LUMINESCENCE AUTOINDUCER SYSTEMS IDENTIFICATION INHIBITION Han, Yin Li, X Qi, Zizhong Zhang, XH Bossier, Peter Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
topic_facet |
Agriculture and Food Sciences cholerae autoinducer 1 N-acylhomoserine lactones autoinducer 2 Edwardsiella tarda quorum sensing VIBRIO-HARVEYI PSEUDOMONAS-AERUGINOSA AEROMONAS-HYDROPHILA GENE-EXPRESSION LUXRI HOMOLOGS LUMINESCENCE AUTOINDUCER SYSTEMS IDENTIFICATION INHIBITION |
description |
Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria. LTB-4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer-2 (AI-2). The AI-2 activity produced by LTB-4 was growth phase dependent and topped at OD600 of 1 center dot 0. The protocol to detect cholerae autoinducer 1 (CAI-1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI-1 activity could not be detected in LTB-4. Conclusion: Edwardsiella tarda LTB-4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL-inducing QS, AI-2 may be the first predominant signal, functioning at early exponential phase. LTB-4 did not produce any CAI-1 activity. Significance and Impact of the Study: Different QS signal molecules of Edw. tarda LTB-4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB-4 produced at least four kinds of AHLs, which seemed to be C-4-HSL, C-6-HSL, 3-oxo-C-6-HSL and an uncharacterized AHL molecule. |
format |
Article in Journal/Newspaper |
author |
Han, Yin Li, X Qi, Zizhong Zhang, XH Bossier, Peter |
author_facet |
Han, Yin Li, X Qi, Zizhong Zhang, XH Bossier, Peter |
author_sort |
Han, Yin |
title |
Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
title_short |
Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
title_full |
Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
title_fullStr |
Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
title_full_unstemmed |
Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4 |
title_sort |
detection of different quorum-sensing signal molecules in a virulent edwardsiella tarda strain ltb-4 |
publishDate |
2010 |
url |
https://biblio.ugent.be/publication/1191499 http://hdl.handle.net/1854/LU-1191499 https://doi.org/10.1111/j.1365-2672.2009.04405.x https://biblio.ugent.be/publication/1191499/file/1191517 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
JOURNAL OF APPLIED MICROBIOLOGY ISSN: 1364-5072 |
op_relation |
https://biblio.ugent.be/publication/1191499 http://hdl.handle.net/1854/LU-1191499 http://dx.doi.org/10.1111/j.1365-2672.2009.04405.x https://biblio.ugent.be/publication/1191499/file/1191517 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1365-2672.2009.04405.x |
container_title |
Journal of Applied Microbiology |
container_volume |
108 |
container_issue |
1 |
container_start_page |
139 |
op_container_end_page |
147 |
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1778533244341321728 |