Detection of different quorum-sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4

Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacteri...

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Bibliographic Details
Published in:Journal of Applied Microbiology
Main Authors: Han, Yin, Li, X, Qi, Zizhong, Zhang, XH, Bossier, Peter
Format: Article in Journal/Newspaper
Language:English
Published: 2010
Subjects:
Agriculture and Food Sciences
cholerae autoinducer 1
N-acylhomoserine lactones
autoinducer 2
Edwardsiella tarda
quorum sensing
VIBRIO-HARVEYI
PSEUDOMONAS-AERUGINOSA
AEROMONAS-HYDROPHILA
GENE-EXPRESSION
LUXRI HOMOLOGS
LUMINESCENCE
AUTOINDUCER
SYSTEMS
IDENTIFICATION
INHIBITION
Scophthalmus maximus
Turbot
Online Access:https://biblio.ugent.be/publication/1191499
http://hdl.handle.net/1854/LU-1191499
https://doi.org/10.1111/j.1365-2672.2009.04405.x
https://biblio.ugent.be/publication/1191499/file/1191517
Description
Summary:Aims: The aim of this study was to elucidate the potential quorum-sensing (QS) signal molecules of an emerging pathogen (Edwardsiella tarda strain LTB-4) of cultured turbot (Scophthalmus maximus). Methods and Results: A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria. LTB-4 was found to have two QS systems, one was based on the AHLs and the other was based on the autoinducer-2 (AI-2). The AI-2 activity produced by LTB-4 was growth phase dependent and topped at OD600 of 1 center dot 0. The protocol to detect cholerae autoinducer 1 (CAI-1) activity in bacteria was modified, lowering the background luminescence of biosensor strain Vibrio harveyi JAF375. CAI-1 activity could not be detected in LTB-4. Conclusion: Edwardsiella tarda LTB-4 produced at least four kinds of AHLs during its whole growth phase. In comparison with the AHL-inducing QS, AI-2 may be the first predominant signal, functioning at early exponential phase. LTB-4 did not produce any CAI-1 activity. Significance and Impact of the Study: Different QS signal molecules of Edw. tarda LTB-4 were clarified by improved bioassays. In contrast to earlier studies detecting two types of AHLs, strain LTB-4 produced at least four kinds of AHLs, which seemed to be C-4-HSL, C-6-HSL, 3-oxo-C-6-HSL and an uncharacterized AHL molecule.

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