Enzymatic Synthesis of Ascorbyl Palmitate in Organic Solvents: Process Optimization and Kinetic Evaluation

This work is focused on the optimization of reaction parameters for the synthesis of ascorbyl palmitate catalyzed by Candida antarctica lipase in different organic solvents. The sequential strategy of experimental designs proved to be useful in maximizing the conditions for product conversion in ter...

Full description

Bibliographic Details
Published in:Food and Bioprocess Technology
Main Authors: Lerin L. A., Richetti A., Dallago R., Treichel H., Mazutti M. A., Oliveira J. V., Antunes O. A. C., Oestreicher E. G., de Oliveira D.
Other Authors: Lerin, L. A., Richetti, A., Dallago, R., Treichel, H., Mazutti, M. A., Oliveira, J. V., Antunes, O. A. C., Oestreicher, E. G., de Oliveira, D.
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
Ascorbyl palmitate
Biocatalysis
Experimental design
Kinetics
Lipase
Neural network
Antarc*
Antarctica
Online Access:http://hdl.handle.net/11392/2464151
https://doi.org/10.1007/s11947-010-0398-1
Description
Summary:This work is focused on the optimization of reaction parameters for the synthesis of ascorbyl palmitate catalyzed by Candida antarctica lipase in different organic solvents. The sequential strategy of experimental designs proved to be useful in maximizing the conditions for product conversion in tert-butanol system using Novozym 435 as catalyst. The optimum production were achieved at ascorbic acid to palmitic acid mole ratio of 1:9, stirring rate of 150 rpm, 70 °C, enzyme concentration of 5 wt.% at 17 h of reaction, resulting in an ascorbyl palmitate conversion of about 67%. Reaction kinetics for ascorbyl palmitate production showed that very satisfactory reaction conversions (~56%) could be achieved in short reaction times (6 h). The kinetic empirical model proposed showed ability to satisfactory represents and predict the experimental data. © 2010 Springer Science + Business Media, LLC.

Similar Items