Effects of seawater alkalinity on calcium and acid-base regulation in juvenile European lobster (Homarus gammarus) during a moult cycle

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record. Fluxes of NH4(+) (acid) and HCO3(-) (base), and whole body calcium content were measured in European lobster (Homarus gammarus) during intermoult (megalopae stage), and during the first 2...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Main Authors: Middlemiss, KL, Urbina, MA, Wilson, RW
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2016
Subjects:
Ammonia excretion
Bicarbonate excretion
Calcification
Carbonate
Crustacean
Exuvia
Moult cycle
European lobster
Homarus gammarus
Online Access:http://hdl.handle.net/10871/20472
https://doi.org/10.1016/j.cbpa.2015.12.002
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Summary:This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record. Fluxes of NH4(+) (acid) and HCO3(-) (base), and whole body calcium content were measured in European lobster (Homarus gammarus) during intermoult (megalopae stage), and during the first 24h for postmoult juveniles under control (~2000μeq/L) and low seawater alkalinity (~830μeq/L). Immediately after moulting, animals lost 45% of the total body calcium via the shed exoskeleton (exuvia), and only 11% was retained in the uncalcified body. At 24h postmoult, exoskeleton calcium increased to ~46% of the intermoult stage. Ammonia excretion was not affected by seawater alkalinity. After moulting, bicarbonate excretion was immediately reversed from excretion to uptake (~4-6 fold higher rates than intermoult) over the whole 24h postmoult period, peaking at 3-6h. These data suggest that exoskeleton calcification is not completed by 24h postmoult. Low seawater alkalinity reduced postmoult bicarbonate uptake by 29% on average. Net acid-base flux (equivalent to net base uptake) followed the same pattern as HCO3(-) fluxes, and was 22% lower in low alkalinity seawater over the whole 24h postmoult period. The common occurrence of low alkalinity in intensive aquaculture systems may slow postmoult calcification in juvenile H. gammarus, increasing the risk of mortalities through cannibalism. The authors would like to acknowledge and thank Dom Boothroyd and Carly Daniels at the National Lobster Hatchery (Padstow, North Cornwall, U.K.) for provision of animals used in this research, and for the valuable comments made by the anonymous reviewers of this manuscript. The analytical equipment used in these experiments were funded through BBSRC and NERC grants to RWW (BB/F009364/1, NE/H010041/1 and BB/J00913X/1).

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