Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion
In the present study, seventy-two adult rats (Rattus norvegicus albinus) aged three months were used. The animals were divided into two groups (control and alcoholic). The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet...
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ftunivespir:oai:repositorio.unesp.br:11449/67259 2023-07-02T03:33:36+02:00 Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion Laura, I. A. Martinez, M. Padovani, C. R. Martinez, F. E. Universidade Estadual Paulista (UNESP) 2003-04-01 167-176 http://hdl.handle.net/11449/67259 http://www.ncbi.nlm.nih.gov/pubmed/12974330 eng eng Journal of Submicroscopic Cytology and Pathology http://www.ncbi.nlm.nih.gov/pubmed/12974330 Journal of Submicroscopic Cytology and Pathology, v. 35, n. 2, p. 167-176, 2003. 1122-9497 http://hdl.handle.net/11449/67259 2-s2.0-2142815344 8727897080522289 1739564105219382 closedAccess Experimental chronic alcoholism Morphometry Ovary Rats Ultrastructure alcohol fat droplet cell vacuole controlled study corpus luteum correlation analysis diet female granulosa cell Leydig cell morphometrics nonhuman rat sugarcane Animalia Arundinaria Rattus Rattus norvegicus Saccharum Saccharum hybrid cultivar info:eu-repo/semantics/article 2003 ftunivespir 2023-06-12T16:14:46Z In the present study, seventy-two adult rats (Rattus norvegicus albinus) aged three months were used. The animals were divided into two groups (control and alcoholic). The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet and sugar-cane liquid (trade 51, 41° Gay Lussac - GL) diluted 30° GL. At the end or 90, 180 and 270 days of treatment, ten rats of each group were anaesthetized with ethyl ether and sacrificed. The ovaries were collected, fixed, included and submitted to analysis by both light and electron microscopy. The alcoholic group showed increase in the number of corpora lutea at both 180 and 270 days of treatment, atresic follicles at 270 days of treatment, decreased diameter of corpora lutea at 180 and 270 days of treatment, the granulosa layer of the antral follicles at 180 days of treatment, and gradual regression of the theca antral follicles. Furthermore, an increase in diameter and posterior regression of the antral follicle were observed, as well as vacuolation, increased lipid droplets in the granulosa cell at 90 days and in the theca at 180 and 270 days of treatment and gradually in the interstitial cell. The rats showed ovarian alterations after ingestion of alcohol. There was a correlation between exposure time to the drug and the injury observed. Article in Journal/Newspaper Rattus rattus Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
institution |
Open Polar |
collection |
Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
op_collection_id |
ftunivespir |
language |
English |
topic |
Experimental chronic alcoholism Morphometry Ovary Rats Ultrastructure alcohol fat droplet cell vacuole controlled study corpus luteum correlation analysis diet female granulosa cell Leydig cell morphometrics nonhuman rat sugarcane Animalia Arundinaria Rattus Rattus norvegicus Saccharum Saccharum hybrid cultivar |
spellingShingle |
Experimental chronic alcoholism Morphometry Ovary Rats Ultrastructure alcohol fat droplet cell vacuole controlled study corpus luteum correlation analysis diet female granulosa cell Leydig cell morphometrics nonhuman rat sugarcane Animalia Arundinaria Rattus Rattus norvegicus Saccharum Saccharum hybrid cultivar Laura, I. A. Martinez, M. Padovani, C. R. Martinez, F. E. Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
topic_facet |
Experimental chronic alcoholism Morphometry Ovary Rats Ultrastructure alcohol fat droplet cell vacuole controlled study corpus luteum correlation analysis diet female granulosa cell Leydig cell morphometrics nonhuman rat sugarcane Animalia Arundinaria Rattus Rattus norvegicus Saccharum Saccharum hybrid cultivar |
description |
In the present study, seventy-two adult rats (Rattus norvegicus albinus) aged three months were used. The animals were divided into two groups (control and alcoholic). The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet and sugar-cane liquid (trade 51, 41° Gay Lussac - GL) diluted 30° GL. At the end or 90, 180 and 270 days of treatment, ten rats of each group were anaesthetized with ethyl ether and sacrificed. The ovaries were collected, fixed, included and submitted to analysis by both light and electron microscopy. The alcoholic group showed increase in the number of corpora lutea at both 180 and 270 days of treatment, atresic follicles at 270 days of treatment, decreased diameter of corpora lutea at 180 and 270 days of treatment, the granulosa layer of the antral follicles at 180 days of treatment, and gradual regression of the theca antral follicles. Furthermore, an increase in diameter and posterior regression of the antral follicle were observed, as well as vacuolation, increased lipid droplets in the granulosa cell at 90 days and in the theca at 180 and 270 days of treatment and gradually in the interstitial cell. The rats showed ovarian alterations after ingestion of alcohol. There was a correlation between exposure time to the drug and the injury observed. |
author2 |
Universidade Estadual Paulista (UNESP) |
format |
Article in Journal/Newspaper |
author |
Laura, I. A. Martinez, M. Padovani, C. R. Martinez, F. E. |
author_facet |
Laura, I. A. Martinez, M. Padovani, C. R. Martinez, F. E. |
author_sort |
Laura, I. A. |
title |
Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
title_short |
Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
title_full |
Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
title_fullStr |
Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
title_full_unstemmed |
Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion |
title_sort |
ultrastructural and morphometric analysis on the ovary of wistar rats after chronic ethanol ingestion |
publishDate |
2003 |
url |
http://hdl.handle.net/11449/67259 http://www.ncbi.nlm.nih.gov/pubmed/12974330 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
Journal of Submicroscopic Cytology and Pathology http://www.ncbi.nlm.nih.gov/pubmed/12974330 Journal of Submicroscopic Cytology and Pathology, v. 35, n. 2, p. 167-176, 2003. 1122-9497 http://hdl.handle.net/11449/67259 2-s2.0-2142815344 8727897080522289 1739564105219382 |
op_rights |
closedAccess |
_version_ |
1770273612729679872 |