Ultrastructural and morphometric analysis on the ovary of Wistar rats after chronic ethanol ingestion

In the present study, seventy-two adult rats (Rattus norvegicus albinus) aged three months were used. The animals were divided into two groups (control and alcoholic). The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet...

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Bibliographic Details
Main Authors: Laura, I. A., Martinez, M., Padovani, C. R., Martinez, F. E.
Other Authors: Universidade Estadual Paulista (UNESP)
Format: Article in Journal/Newspaper
Language:English
Published: 2003
Subjects:
Experimental chronic alcoholism
Morphometry
Ovary
Rats
Ultrastructure
alcohol
fat droplet
cell vacuole
controlled study
corpus luteum
correlation analysis
diet
female
granulosa cell
Leydig cell
morphometrics
nonhuman
rat
sugarcane
Animalia
Arundinaria
Rattus
Rattus norvegicus
Saccharum
Saccharum hybrid cultivar
Rattus rattus
Online Access:http://hdl.handle.net/11449/67259
http://www.ncbi.nlm.nih.gov/pubmed/12974330
Description
Summary:In the present study, seventy-two adult rats (Rattus norvegicus albinus) aged three months were used. The animals were divided into two groups (control and alcoholic). The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet and sugar-cane liquid (trade 51, 41° Gay Lussac - GL) diluted 30° GL. At the end or 90, 180 and 270 days of treatment, ten rats of each group were anaesthetized with ethyl ether and sacrificed. The ovaries were collected, fixed, included and submitted to analysis by both light and electron microscopy. The alcoholic group showed increase in the number of corpora lutea at both 180 and 270 days of treatment, atresic follicles at 270 days of treatment, decreased diameter of corpora lutea at 180 and 270 days of treatment, the granulosa layer of the antral follicles at 180 days of treatment, and gradual regression of the theca antral follicles. Furthermore, an increase in diameter and posterior regression of the antral follicle were observed, as well as vacuolation, increased lipid droplets in the granulosa cell at 90 days and in the theca at 180 and 270 days of treatment and gradually in the interstitial cell. The rats showed ovarian alterations after ingestion of alcohol. There was a correlation between exposure time to the drug and the injury observed.

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