Sub-lethal combined effects of illicit drug and decreased pH on marine mussels: A short-time exposure to crack cocaine in CO2 enrichment scenarios

• Published in: Marine Pollution Bulletin
• Main Authors: da Silva Souza, Lorena, Bonnail, Estefanía, Maranho, Luciane Alves, Pusceddu, Fabio Hermes, Cortez, Fernando Sanzi, Cesar, Augusto, Ribeiro, Daniel Araki, Riba, Inmaculada, de Souza Abessa, Denis M., DelValls, Ángel, Pereira, Camilo Dias Seabra
• Other Authors: Universidade Estadual Paulista (UNESP)
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2021
• Subjects: Biomarkers; CO2 enrichment; Crack cocaine; Marine mussel; Ocean acidification
• Online Access: http://hdl.handle.net/11449/231479; https://doi.org/10.1016/j.marpolbul.2021.112735

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Processo FAPESP: 2018/18456-4 CNPq: 409187/2016-0 The increasing CO2-concentrations in the atmosphere promote ocean acidification. Seawater chemistry changes interact with contaminants, such as illicit drugs in the coastal zones. This work evaluates impacts of pH decrease and crack-cocaine exposure on the commercial mussel Perna perna through biomarker responses (lysosomal membrane stability, lipid peroxidation, and DNA strand breaks). The organisms were exposed to different crack-cocaine concentrations (0.5, 5.0, and 50 μg L−1) combined with different pH values (8.3, 8.0, 7.5, 7.0, 6.5, and 6.0) for 96 h. Crack-cocaine in the different acidification scenarios triggered cyto-genotoxicity, which affected the overall health of mussels exposed to cocaine environmentally relevant concentration. This study produced the first data on biomarker responses associated with CO2-induced acidification and illicit drugs (crack-cocaine) in marine organisms.