Potencial biotecnológico de fungos marinhos e antárticos da central de recursos microbianos da UNESP (CRM-UNESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC Microbial culture collections have an important contribution to the preservation, maintenance, and availability of the microbial material for academic and research...
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| Format: | Master Thesis |
| Language: | Portuguese |
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Universidade Estadual Paulista (Unesp)
2015
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| Online Access: | http://hdl.handle.net/11449/134176 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/25-01-2016/000857377.pdf |
| Summary: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC Microbial culture collections have an important contribution to the preservation, maintenance, and availability of the microbial material for academic and research pourpose. The mission of the Central of Microbial Resources of the UNESP (CRM-UNESP) is to provide support for the knowledge of the microbial biodiversity, as well as for the scientific and technological development, supporting relevant projects. CRM-UNESP has an associated collection of filamentous fungi and yeast from uncommon and/or extreme environments. In this context, the present study aims to reorganize the marine-derived fungal collection from brasilian coast and the marine and terrestrial collection from Antarctica and to prospect the enzymes xylanase and L-asparaginase. For that end, about 696 marine fungi originating from brasilian coast and 185 isolated from antarctic environments had their viability and purity assessed and were photographed and preserved by two different methods: criopreservation and Castellani. Among them, 71% isolated were considered pure and viable and were submitted to the quantitative and qualitative screening for the production of xylanase and L-asparaginase. In the qualitative quantitative screening (solid medium) 112 filamentous fungi able to produce xilanases were isolated from marine environment (brasilian coast) and 19 from the antartic environment. These isolates were submitted to quantitative screening (liquid medium), in which, the fungus LAMAI 31 indentified as Aspergillus tubingensis presented the best result of enzymatic production (49.41 U/mL). For L-asparaginase besides the 622 isolates, 17 additional filamentous fungi were analysed, being 510 originated from the brasilian marine coast and 129 from Antarctica. Among them, 407 fungi were positive for L-asparaginase in solid medium and were submitted to the screening in liquid medium. L-asparaginase was produced in a ... |
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