Positional assembly of enzymes in polymersome nanoreactors for cascade reactions

In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combinati...

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Bibliographic Details
Main Authors: Vriezema, DM, Garcia, PML, Sancho Oltra, N, Hatzakis, NS, Kuiper, SM, Nolte, RJM Roeland, Rowan, Alan E, Hest, JCM Jan van
Format: Article in Journal/Newspaper
Language:English
Published: 2007
Subjects:
Antarc*
Antarctica
Online Access:http://repository.tue.nl/882239
Description
Summary:In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)). (Chemical Equation Presented)

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