The effect of water concentration on the activity and stability of CLECs in supercritical CO2 in continuous operation
In this study the effect of the water concn. on a crystd. enzyme of Candida antarctica lipase B (ChiroCLEC-CAB) in supercrit. carbon dioxide (scCO2) is studied. The model reaction used is the enantioselective esterification of racemic 1-Ph ethanol with vinyl acetate; the reaction is performed in scC...
| Main Authors: | , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
2006
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| Subjects: | |
| Online Access: | http://repository.tue.nl/621729 |
| Summary: | In this study the effect of the water concn. on a crystd. enzyme of Candida antarctica lipase B (ChiroCLEC-CAB) in supercrit. carbon dioxide (scCO2) is studied. The model reaction used is the enantioselective esterification of racemic 1-Ph ethanol with vinyl acetate; the reaction is performed in scCO2 at 40 DegC and 90 bar in batch and in continuous operation. Kinetic parameters have been derived from continuous expts., leading to a catalytic turnover no. of 0.95 s-1. The optimum activity is reached at low water concns. (0.05 g L-1). At lower concns., CO2 is stripping water from the enzyme leading to deactivation. However, adding a small amt. of water to the substrates can reverse this deactivation and the enzyme activity is restored. [on SciFinder (R)] |
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