In vivo and in vitro evolution of the effects of cypermethrin on turbot (Scophthalmus maximus, Linnaeus, 1758) spermatozoa

Synthetic pyrethroid pesticide is commonly used in agricultural activities in the Black Sea region during reproduction period of turbot. In this sense, in vivo and in vitro studies have shown that cypermethrin (CYP) could be one of the environmental factors affecting decreasing turbot stocks. In thi...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Main Authors: Saka, Sahin, Beken, Atife Tuba, Aydin, Ilhan, Firat, Kursat, Suzer, Cuneyt, Benzer, Fulya, Erisir, Mine
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Science Inc 2022
Subjects:
Histopathological changes
Male Reproductive-System
Sperm Motility
Oxidative Stress
Pyrethroid Insecticides
Lipid-Peroxidation
Seminal Plasma
Common Carp
Dna-Damage
Fish
Antioxidant
Turbot
Scopthalmus maximus
Cypermethrin
Sperm kinematics
Antioxidant activity
Ege
Scophthalmus maximus
Online Access:https://hdl.handle.net/11454/76450
https://doi.org/10.1016/j.cbpc.2022.109298
Description
Summary:Synthetic pyrethroid pesticide is commonly used in agricultural activities in the Black Sea region during reproduction period of turbot. In this sense, in vivo and in vitro studies have shown that cypermethrin (CYP) could be one of the environmental factors affecting decreasing turbot stocks. In this study, effects of in vivo and in vitro administration of CYP, a synthetic pyrethroid, on sperm kinematics motility (MOT), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straight line velocity (STR), amplitude of lateral head (ALH), beat cross frequency (BCF), oxidative stress biomarkers malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) and also histopathological alterations in gonads were investigated in spermatazoa of turbot (Schopthalmus maximus). Broodstock was supplied from culture origin and used in spawning season, additionally, two (0, 0.187 and 0.218 ppb) and three (0, 1.025, 2.05 and 4.1 ppb) different CYP concentrations were performed for in vivo and in vitro studies, respectively. In vivo and in vitro studies, significant reductions were found in sperm MOT, PM, VCL, VSL, VAP, LIN, and ALH properties depend on the increase in CYP concentrations (p 0.05). Besides, activities of GSH, GPx, SOD, and CAT increased. In terms of histological alterations, no difference was observed among groups (0, 0.187 and 0.218 ppb) in the maturity stage of the germ cells. According to obtained results, sperm kinematics was affected significantly with increased the dose levels of CYP (p 0.05). Funding This work was supported by the Ege University Scientific Research Projects Coordination [grant numbers 16SUF032] . Ege University Scientific Research Projects Coordination [16SUF032]

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