A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage
Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of t...
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Language: | English |
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ftunivctemuco:oai:repositoriodigital.uct.cl:10925/4241 2023-05-15T15:32:20+02:00 A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage Beltran, Jorge F. Zamorano, Mauricio Herrera Belen, Lisandra Risopatron, Jennie Valdebenito, Ivan Norambuena, Juan-Alejandro Farias, Joaquin A. Farias, Jorge G. 2021-10-04T18:44:48Z http://repositoriodigital.uct.cl/handle/10925/4241 https://doi.org/10.1016/j.aquaculture.2021.737211 en eng ELSEVIER AQUACULTURE,Vol.545,,2021 http://repositoriodigital.uct.cl/handle/10925/4241 doi:10.1016/j.aquaculture.2021.737211 AQUACULTURE Salmo salar Motility Proteomics Channel Calcium Article 2021 ftunivctemuco https://doi.org/10.1016/j.aquaculture.2021.737211 2021-10-09T23:50:40Z Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Académico de la Universidad Católica de Temuco (UCT) Aquaculture 545 737211 |
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Repositorio Académico de la Universidad Católica de Temuco (UCT) |
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ftunivctemuco |
language |
English |
topic |
Salmo salar Motility Proteomics Channel Calcium |
spellingShingle |
Salmo salar Motility Proteomics Channel Calcium Beltran, Jorge F. Zamorano, Mauricio Herrera Belen, Lisandra Risopatron, Jennie Valdebenito, Ivan Norambuena, Juan-Alejandro Farias, Joaquin A. Farias, Jorge G. A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
topic_facet |
Salmo salar Motility Proteomics Channel Calcium |
description |
Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation. |
format |
Article in Journal/Newspaper |
author |
Beltran, Jorge F. Zamorano, Mauricio Herrera Belen, Lisandra Risopatron, Jennie Valdebenito, Ivan Norambuena, Juan-Alejandro Farias, Joaquin A. Farias, Jorge G. |
author_facet |
Beltran, Jorge F. Zamorano, Mauricio Herrera Belen, Lisandra Risopatron, Jennie Valdebenito, Ivan Norambuena, Juan-Alejandro Farias, Joaquin A. Farias, Jorge G. |
author_sort |
Beltran, Jorge F. |
title |
A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
title_short |
A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
title_full |
A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
title_fullStr |
A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
title_full_unstemmed |
A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage |
title_sort |
differential proteomic study reveals the downregulation of several plasma membrane ca2+-binding proteins in the sperm of atlantic salmon (salmo salar) following cold storage |
publisher |
ELSEVIER |
publishDate |
2021 |
url |
http://repositoriodigital.uct.cl/handle/10925/4241 https://doi.org/10.1016/j.aquaculture.2021.737211 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
AQUACULTURE |
op_relation |
AQUACULTURE,Vol.545,,2021 http://repositoriodigital.uct.cl/handle/10925/4241 doi:10.1016/j.aquaculture.2021.737211 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2021.737211 |
container_title |
Aquaculture |
container_volume |
545 |
container_start_page |
737211 |
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1766362842297008128 |