A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage

Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of t...

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Bibliographic Details
Published in:Aquaculture
Main Authors: Beltran, Jorge F., Zamorano, Mauricio, Herrera Belen, Lisandra, Risopatron, Jennie, Valdebenito, Ivan, Norambuena, Juan-Alejandro, Farias, Joaquin A., Farias, Jorge G.
Format: Article in Journal/Newspaper
Language:English
Published: ELSEVIER 2021
Subjects:
Salmo salar
Motility
Proteomics
Channel
Calcium
Atlantic salmon
Online Access:http://repositoriodigital.uct.cl/handle/10925/4241
https://doi.org/10.1016/j.aquaculture.2021.737211
Description
Summary:Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation.

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