Temperature differentially affects gene expression in antarctic thraustochytrid oblongichytrium sp. RT2316-13

Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid's response to a decrea...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Paredes, Paris, Larama, Giovanni, Flores, Liset, Leyton, Allison, Ili, Carmen Gloria, Asenjo De Leuze De Lancizolle, Juan Alfonso, Chisti, Yusuf, Shene, Carolina
Format: Article in Journal/Newspaper
Language:English
Published: MDPI 2020
Subjects:
Gene expression analysis
Lipid metabolism
Oblongichytrium sp
Polyunsaturated fatty acid synthesis
Thraustochytrids
Antarctic
Inach
Antarc*
antartic*
Instituto Antartico Chileno
Online Access:https://doi.org/10.3390/md18110563
https://repositorio.uchile.cl/handle/2250/179759
Description
Summary:Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid's response to a decrease in growth temperature from 15 degrees C to 5 degrees C. Around 4% (2944) of the genes were differentially expressed (DE) and only a few of the DE genes (533 upregulated; 206 downregulated) had significant matches to those in the SwissProt database. Most of the annotated DE genes were related to cell membrane composition (fatty acids, sterols, phosphatidylinositol), the membrane enzymes linked to cell energetics, and membrane structure (cytoskeletal proteins and enzymes). In RT2316-13, the synthesis of long-chain polyunsaturated fatty acids occurred through omega 3- and omega 6-pathways. Enzymes of the alternative pathways (Delta 8-desaturase and Delta 9-elongase) were also expressed. The upregulation of the genes coding for a Delta 5-desaturase and a Delta 5-elongase involved in the synthesis of EPA and DHA, explained the enrichment of total lipid with these two long-chain fatty acids at the low temperature. This molecular response has the potential to be used for producing microbial lipids with a fatty acids profile similar to that of fish oils. Instituto Antartico Chileno (INACH), project Inach RT 23-16 Conicyt, project Centre for Biotechnology and Bioengineering (CeBiB) FB-0001

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