Molecular Analysis of Bacterial Microbiota Associated with Oysters (Crassostrea gigas and Crassostrea corteziensis) in Different Growth Phases at Two Cultivation Sites

Microbiota presumably plays an essential role in inhibiting pathogen colonization and in the maintenance of health in oysters, but limited data exist concerning their different growth phases and conditions. We analyzed the bacterial microbiota composition of two commercial oysters: Crassostrea gigas...

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Bibliographic Details
Published in:Microbial Ecology
Main Authors: Trabal, Natalia, Mazón Suástegui, José M., Vázquez Juárez, Ricardo, Asencio-Valle, Felipe, Morales-Bojórquez, Enrique, Romero Ormazábal, Jaime
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
Ecology
Evolution
Behavior and Systematics
Soil Science
Crassostrea gigas
Online Access:https://doi.org/10.1007/s00248-012-0039-5
https://repositorio.uchile.cl/handle/2250/165642
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Summary:Microbiota presumably plays an essential role in inhibiting pathogen colonization and in the maintenance of health in oysters, but limited data exist concerning their different growth phases and conditions. We analyzed the bacterial microbiota composition of two commercial oysters: Crassostrea gigas and Crassostrea corteziensis. Differences in microbiota were assayed in three growth phases: post-larvae at the hatchery, juvenile, and adult at two grow-out cultivation sites. Variations in the microbiota were assessed by PCR analysis of the 16S rRNA gene in DNA extracted from depurated oysters. Restriction fragment length polymorphism (RFLP) profiles were studied using Dice's similarity coefficient (Cs) and statistical principal component analysis (PCA). The microbiota composition was determined by sequencing temperature gradient gel electrophoresis (TGGE) bands. The RFLP analysis of post-larvae revealed homology in the microbiota of both oyster species (Cs > 88 %). Dice and PCA analyses

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