Anticuerpos policlonales antiinmunoglobulinas de salmón; herramientas potenciales para diagnóstico

Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Igs. This antibody...

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Bibliographic Details
Main Authors: Aguillón Gutiérrez, Juan Carlos, Smith, P., Colombo, A., Molina, M. C., Ferreira, A.
Format: Article in Journal/Newspaper
Language:unknown
Published: 1997
Subjects:
Diagnosis
Polyclonal antibodies
Salmon
Atlantic salmon
Salmo salar
Online Access:https://repositorio.uchile.cl/handle/2250/157257
Description
Summary:Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Igs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes.

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