Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius),...
Published in: | Fishes |
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Main Authors: | , , , , , , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
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Subjects: | |
Online Access: | http://hdl.handle.net/10317/11926 https://doi.org/10.3390/fishes7030117 https://www.mdpi.com/2410-3888/7/3/117 |
Summary: | In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. This research was funded by Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia through the project “Mejora de la competitividad del sector de la corvina a través de la selección genética (GENECOR, 21002/PI/18)” in the call for grants for projects for the development of scientific and technical research by competitive groups, included in the Regional Program for the Promotion of Scientific and Technical Research (Action Plan 2018). A.V. was funded by a pre-doctora research fellowship (20716/FPI/18. Fundación Séneca. Cofinanciado por grupo Avramar S.L. Región de Murcia (Spain)). The Avramar S.L. group provided financial support and the use of its facilities for this study. |
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