Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase

• Main Authors: Delsaute, Maud, Berlemont, Renaud, Dehareng, Dominique, Van Elder, Dany, Galleni, Moreno, Bauvois, Cédric
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: Physique de l'état condense [supraconducteur]; Physique de l'état condense [struct. propr. thermiques; etc.]; Physique de l'état condense [struct. électronique; Biophysique; Biologie; Biologie moléculaire; Biochimie; bacterial cellulose synthesis; endoglucanases; family 5 glycosyl hydrolases; psychrotolerant; uncultured bacteria; Antarctic; The Antarctic; Antarc*
• Online Access: http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/168544

RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH55) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4Å resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH55 subfamily members. © 2013 International Union of Crystallography. SCOPUS: ar.j info:eu-repo/semantics/published