Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

• Published in: Aquaculture
• Main Authors: Prieto, Daniel, Markaide, Pablo, Urrutxurtu, Inaki, Navarro, Enrique, Artigaud, Sébastien, Fleury, Elodie, Ibarrola, Irrintzi, Urrutia, Miren Bego
• Other Authors: Universidad del Pais Vasco / Euskal Herriko Unibertsitatea Espagne (UPV/EHU), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2019
• Subjects: ACL; Transcriptome; pacific oyster; identification; gene-expression; Gill; oyster crassostrea-gigas; growth-rate; blue mussel; Fast-growing; Mussel; myostatin gene; physiological-basis; protein; stress; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Pacific; Krebs; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.science/hal-02874960; https://hal.science/hal-02874960/document; https://hal.science/hal-02874960/file/65293.pdf; https://doi.org/10.1016/j.aquaculture.2019.734242

WOS:000480233600050 The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: F-BP, S-BP, F-AP and S-AP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast- and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas bio-synthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system ...