Identification and quantification of phosphorus in cheeses - methodological investigations by solid-state 31P NMR spectroscopy

• Main Authors: Rondeau-Mouro, Corinne, C., Gobet, Mallory, Mietton, Bernard, Buchin, S, Moreau, Céline
• Other Authors: Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), FLAveur, VIsion et Comportement du consommateur (FLAVIC), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Ecole Nationale d'Industrie Laitière et des Biotechnologies (ENILBIO), Unité de recherches en Technologie et Analyses Laitières (URTAL)
• Format: Conference Object
• Language: English
• Published: HAL CCSD 2008
• Subjects: SPECTROSCOPY; PHOSPHORUS; CHEESE; SOLID-STATE 31P NMR; [SDV.IDA]Life Sciences [q-bio]/Food engineering; [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering; Reykjavík; Iceland
• Online Access: https://hal.inrae.fr/hal-02756831

International audience NMR has been shown to be an interesting tool for investigating dairy foods, in particular cheese products, for which the method is non-destructive and well suited for investigating their compounds showing different structure and mobility during ripening. Solid-state magic angle spinning 31P NMR spectroscopy was used to characterize the structure and composition of semi-hard cheeses. Assignment including overlapping resonances from mobile/immobile phosphorylated serine residues and inorganic calcium phosphates, has been realised using different NMR techniques (spin echo, cross polarisation and single pulse excitation) and by comparison with spectra of literature. Studies at various ripening stages and salt contents have been achieved in order to understand the impact of salt on the phosphates structures and mobility within the cheese matrix, as well as its influence in enzymatic activities.