| Summary: | Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) possess two paralogous IgM heavy chain (μ) genes related to ancestral tetraploidy. Accordingly, IgM subpopulations of Atlantic salmon and brown trout can be separated by gradient anion exchange chromatography (AEC) into two distinct peaks. In contrast, IgM of arctic char (Salvelinus alpinus) and rainbow trout (Oncorhynchus mykiss) is eluted in a single peak. In the present study mass spectrometry analysis verified that IgM of peak 1 (subpopulation 1) have heavy chains previously designated as μB type whereas IgM of peak 2 (subpopulation 2) have heavy chains of μA type, in Atlantic salmon and brown trout. Salmon IgM of both peak 1 and peak 2 contain light chains of the two most common isotypes: IgL1 and IgL3. Two adjacent cysteine residues are present near the C-terminal part of μB, in contrast to one cysteine residue in μA. Most likely, the additional cysteine is involved in inter-chain disulfide bonding and influences the elution profiles of IgM-A and IgM-B on AEC. Molecular cloning of μ cDNA from arctic char revealed two sub-variants (μA-1 and μA-2), and hybrids of char/salmon expressed μA-1, μA-2, μA and μB, indicating that there are two paralogous μ loci in the haploid genome of char, like in Atlantic salmon. Neither of the μ sub-variants in arctic char have the additional cysteine, and char IgM, as well as salmon and brown trout IgM-A, show a lower degree of inter-chain disulfide bonding than IgM-B when subjected to denaturation and gel electrophoresis under non-reducing conditions. Surprisingly, a monoclonal antibody MAb4C10 against rainbow trout IgM, reacted with μA in salmon, whereas in brown trout it reacted with μB. MAb4C10 was conjugated to magnetic beads and used to separate cells, demonstrating that μ transcripts residing from captured cells were primarily of A type in salmon and B type in brown trout. It is plausible to assume that DNA has been exchanged between the paralogous A and B loci during evolution while maintaining the two ...
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