Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon
A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJT, was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did...
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Online Access: | https://hdl.handle.net/1956/15237 https://doi.org/10.1007/s10482-015-0630-0 |
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ftunivbergen:oai:bora.uib.no:1956/15237 2023-05-15T15:31:42+02:00 Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon Småge, Sverre Bang Brevik, Øyvind Jakobsen Duesund, Henrik Ottem, Karl Fredrik Watanabe, Kuninori Nylund, Are 2016-11-04T09:16:28Z application/pdf https://hdl.handle.net/1956/15237 https://doi.org/10.1007/s10482-015-0630-0 eng eng Springer urn:issn:0003-6072 https://hdl.handle.net/1956/15237 https://doi.org/10.1007/s10482-015-0630-0 cristin:1333678 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2015 The Author(s) Norway Polyphasic taxonomy Salmon farming Skin lesions Ulcerative disease Winter ulcers Peer reviewed Journal article 2016 ftunivbergen https://doi.org/10.1007/s10482-015-0630-0 2023-03-14T17:43:22Z A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJT, was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did not adhere to the agar. The 16S rRNA gene sequence showed that the strain belongs to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Strain HFJT exhibits high 16S rRNA gene sequence similarity values to Tenacibaculum dicentrarchi NCIMB 14598T (97.2 %). The strain was found to grow at 2–20 °C and only in the presence of sea salts. The respiratory quinone was identified as menaquinone 6 and the major fatty acids were identified as summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0, anteiso-C15:0, iso-C15:1 and iso-C15:0 3-OH. The DNA G+C content was determined to be 34.1 mol%. DNA–DNA hybridization and comparative phenotypic and genetic tests were performed with the phylogenetically closely related type strains, T. dicentrarchi NCIMB 14598T and Tenacibaculumovolyticum NCIMB 13127T. These data, as well as phylogenetic analyses, suggest that strain HFJT should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum finnmarkense sp. nov. is proposed; the type strain is HFJ T = (DSM 28541T = NCIMB 42386T). publishedVersion Article in Journal/Newspaper Atlantic salmon Finnmark Salmo salar Finnmark University of Bergen: Bergen Open Research Archive (BORA-UiB) Norway Antonie van Leeuwenhoek 109 2 273 285 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Norway Polyphasic taxonomy Salmon farming Skin lesions Ulcerative disease Winter ulcers |
spellingShingle |
Norway Polyphasic taxonomy Salmon farming Skin lesions Ulcerative disease Winter ulcers Småge, Sverre Bang Brevik, Øyvind Jakobsen Duesund, Henrik Ottem, Karl Fredrik Watanabe, Kuninori Nylund, Are Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
topic_facet |
Norway Polyphasic taxonomy Salmon farming Skin lesions Ulcerative disease Winter ulcers |
description |
A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJT, was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did not adhere to the agar. The 16S rRNA gene sequence showed that the strain belongs to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Strain HFJT exhibits high 16S rRNA gene sequence similarity values to Tenacibaculum dicentrarchi NCIMB 14598T (97.2 %). The strain was found to grow at 2–20 °C and only in the presence of sea salts. The respiratory quinone was identified as menaquinone 6 and the major fatty acids were identified as summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0, anteiso-C15:0, iso-C15:1 and iso-C15:0 3-OH. The DNA G+C content was determined to be 34.1 mol%. DNA–DNA hybridization and comparative phenotypic and genetic tests were performed with the phylogenetically closely related type strains, T. dicentrarchi NCIMB 14598T and Tenacibaculumovolyticum NCIMB 13127T. These data, as well as phylogenetic analyses, suggest that strain HFJT should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum finnmarkense sp. nov. is proposed; the type strain is HFJ T = (DSM 28541T = NCIMB 42386T). publishedVersion |
format |
Article in Journal/Newspaper |
author |
Småge, Sverre Bang Brevik, Øyvind Jakobsen Duesund, Henrik Ottem, Karl Fredrik Watanabe, Kuninori Nylund, Are |
author_facet |
Småge, Sverre Bang Brevik, Øyvind Jakobsen Duesund, Henrik Ottem, Karl Fredrik Watanabe, Kuninori Nylund, Are |
author_sort |
Småge, Sverre Bang |
title |
Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
title_short |
Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
title_full |
Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
title_fullStr |
Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
title_full_unstemmed |
Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon |
title_sort |
tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family flavobacteriaceae isolated from atlantic salmon |
publisher |
Springer |
publishDate |
2016 |
url |
https://hdl.handle.net/1956/15237 https://doi.org/10.1007/s10482-015-0630-0 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Finnmark Salmo salar Finnmark |
genre_facet |
Atlantic salmon Finnmark Salmo salar Finnmark |
op_relation |
urn:issn:0003-6072 https://hdl.handle.net/1956/15237 https://doi.org/10.1007/s10482-015-0630-0 cristin:1333678 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2015 The Author(s) |
op_doi |
https://doi.org/10.1007/s10482-015-0630-0 |
container_title |
Antonie van Leeuwenhoek |
container_volume |
109 |
container_issue |
2 |
container_start_page |
273 |
op_container_end_page |
285 |
_version_ |
1766362222563426304 |