Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon

A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJT, was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek
Main Authors: Småge, Sverre Bang, Brevik, Øyvind Jakobsen, Duesund, Henrik, Ottem, Karl Fredrik, Watanabe, Kuninori, Nylund, Are
Format: Article in Journal/Newspaper
Language:English
Published: Springer 2016
Subjects:
Norway
Polyphasic taxonomy
Salmon farming
Skin lesions
Ulcerative disease
Winter ulcers
Atlantic salmon
Finnmark
Salmo salar
Online Access:https://hdl.handle.net/1956/15237
https://doi.org/10.1007/s10482-015-0630-0
Description
Summary:A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJT, was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did not adhere to the agar. The 16S rRNA gene sequence showed that the strain belongs to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Strain HFJT exhibits high 16S rRNA gene sequence similarity values to Tenacibaculum dicentrarchi NCIMB 14598T (97.2 %). The strain was found to grow at 2–20 °C and only in the presence of sea salts. The respiratory quinone was identified as menaquinone 6 and the major fatty acids were identified as summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0, anteiso-C15:0, iso-C15:1 and iso-C15:0 3-OH. The DNA G+C content was determined to be 34.1 mol%. DNA–DNA hybridization and comparative phenotypic and genetic tests were performed with the phylogenetically closely related type strains, T. dicentrarchi NCIMB 14598T and Tenacibaculumovolyticum NCIMB 13127T. These data, as well as phylogenetic analyses, suggest that strain HFJT should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum finnmarkense sp. nov. is proposed; the type strain is HFJ T = (DSM 28541T = NCIMB 42386T). publishedVersion

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