Purification and Characterization of the tricarboxylate carrier from Eel liver mitochondria

• Main Authors: V. ZARA, L. SICULELLA, G. GNONI AND F. PALMIERI, IACOBAZZI, Vito
• Other Authors: V., Zara, Iacobazzi, Vito, L., Siculella, G. GNONI AND F., Palmieri
• Format: Article in Journal/Newspaper
• Language: English
• Published: 1996
• Subjects: purification; carrier; mitochondria; citrate; Triton; Anguilla anguilla
• Online Access: http://hdl.handle.net/11586/42965

The tricarboxylate carrier from eel (Anguilla anguilla) liver mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and Matrex Gel Blue B. On SDS-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an ap...