Trapping and imaging of micron-sized embryos using dielectrophoresis.

Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interac...

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Bibliographic Details
Published in:ELECTROPHORESIS
Main Authors: Khoshmanesh, K, Kiss, N, Nahavandi, S, Evans, CW, Cooper, JM, Williams, DE, Wlodkowic, D
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
Antarctic
Antarc*
Online Access:http://hdl.handle.net/2292/9698
https://doi.org/10.1002/elps.201100160
Description
Summary:Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force.

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