Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica

The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from...

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Published in:Environmental Microbiology
Main Authors: Ayton, J, Aislabie, J, Barker, GM, Saul, D, Turner, S
Format: Article in Journal/Newspaper
Language:English
Published: WILEY-BLACKWELL PUBLISHING, INC 2010
Subjects:
Online Access:http://hdl.handle.net/2292/7916
https://doi.org/10.1111/j.1462-2920.2009.02111.x
id ftunivauckland:oai:researchspace.auckland.ac.nz:2292/7916
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spelling ftunivauckland:oai:researchspace.auckland.ac.nz:2292/7916 2023-05-15T13:59:29+02:00 Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica Ayton, J Aislabie, J Barker, GM Saul, D Turner, S 2010 http://hdl.handle.net/2292/7916 https://doi.org/10.1111/j.1462-2920.2009.02111.x EN eng WILEY-BLACKWELL PUBLISHING, INC ENVIRON MICROBIOL Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1462-2912/ https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm Copyright: 2009 Society for Applied Microbiology and Blackwell Publishing Ltd. http://purl.org/eprint/accessRights/RestrictedAccess http://dx.doi.org/10.1111/j.1462-2920.2009.02111.x IN-SITU HYBRIDIZATION PHYLOGENETIC ANALYSIS BACTERIAL DIVERSITY BOREAL FOREST VICTORIA LAND KINGDOM CRENARCHAEOTA MICROBIAL COMMUNITIES ARCHAEAL ASSEMBLAGES PLANT-ROOTS ABUNDANCE Journal Article 2010 ftunivauckland https://doi.org/10.1111/j.1462-2920.2009.02111.x 2013-01-22T00:19:47Z The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from four locations. A total of 1452 clones were analysed by restriction fragment length polymorphism and assigned to 43 operational taxonomic units from which representatives were sequenced. Archaea were primarily restricted to coastal mineral soils which showed a predominance of Crenarchaeota belonging to group 1.1b (> 99% of clones). These clones were assigned to six clusters (A through F), based on shared identity to sequences in the GenBank database. Ordination indicated that soil chemistry and water content determined archaeal community structure. This is the first comprehensive study of the archaeal community in Antarctic soils and as such provides a reference point for further investigation of microbial function in this environment. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea Victoria Land University of Auckland Research Repository - ResearchSpace Antarctic Ross Sea Victoria Land Environmental Microbiology 12 3 689 703
institution Open Polar
collection University of Auckland Research Repository - ResearchSpace
op_collection_id ftunivauckland
language English
topic IN-SITU HYBRIDIZATION
PHYLOGENETIC ANALYSIS
BACTERIAL DIVERSITY
BOREAL FOREST
VICTORIA LAND
KINGDOM CRENARCHAEOTA
MICROBIAL COMMUNITIES
ARCHAEAL ASSEMBLAGES
PLANT-ROOTS
ABUNDANCE
spellingShingle IN-SITU HYBRIDIZATION
PHYLOGENETIC ANALYSIS
BACTERIAL DIVERSITY
BOREAL FOREST
VICTORIA LAND
KINGDOM CRENARCHAEOTA
MICROBIAL COMMUNITIES
ARCHAEAL ASSEMBLAGES
PLANT-ROOTS
ABUNDANCE
Ayton, J
Aislabie, J
Barker, GM
Saul, D
Turner, S
Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
topic_facet IN-SITU HYBRIDIZATION
PHYLOGENETIC ANALYSIS
BACTERIAL DIVERSITY
BOREAL FOREST
VICTORIA LAND
KINGDOM CRENARCHAEOTA
MICROBIAL COMMUNITIES
ARCHAEAL ASSEMBLAGES
PLANT-ROOTS
ABUNDANCE
description The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from four locations. A total of 1452 clones were analysed by restriction fragment length polymorphism and assigned to 43 operational taxonomic units from which representatives were sequenced. Archaea were primarily restricted to coastal mineral soils which showed a predominance of Crenarchaeota belonging to group 1.1b (> 99% of clones). These clones were assigned to six clusters (A through F), based on shared identity to sequences in the GenBank database. Ordination indicated that soil chemistry and water content determined archaeal community structure. This is the first comprehensive study of the archaeal community in Antarctic soils and as such provides a reference point for further investigation of microbial function in this environment.
format Article in Journal/Newspaper
author Ayton, J
Aislabie, J
Barker, GM
Saul, D
Turner, S
author_facet Ayton, J
Aislabie, J
Barker, GM
Saul, D
Turner, S
author_sort Ayton, J
title Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
title_short Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
title_full Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
title_fullStr Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
title_full_unstemmed Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica
title_sort crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the ross sea region of antarctica
publisher WILEY-BLACKWELL PUBLISHING, INC
publishDate 2010
url http://hdl.handle.net/2292/7916
https://doi.org/10.1111/j.1462-2920.2009.02111.x
geographic Antarctic
Ross Sea
Victoria Land
geographic_facet Antarctic
Ross Sea
Victoria Land
genre Antarc*
Antarctic
Antarctica
Ross Sea
Victoria Land
genre_facet Antarc*
Antarctic
Antarctica
Ross Sea
Victoria Land
op_source http://dx.doi.org/10.1111/j.1462-2920.2009.02111.x
op_relation ENVIRON MICROBIOL
op_rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1462-2912/
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
Copyright: 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.
http://purl.org/eprint/accessRights/RestrictedAccess
op_doi https://doi.org/10.1111/j.1462-2920.2009.02111.x
container_title Environmental Microbiology
container_volume 12
container_issue 3
container_start_page 689
op_container_end_page 703
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