Crenarchaeota affiliated with group 1.1b are prevalent in coastal mineral soils of the Ross Sea region of Antarctica

The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from...

Full description

Bibliographic Details
Published in:Environmental Microbiology
Main Authors: Ayton, J, Aislabie, J, Barker, GM, Saul, D, Turner, S
Format: Article in Journal/Newspaper
Language:English
Published: WILEY-BLACKWELL PUBLISHING, INC 2010
Subjects:
IN-SITU HYBRIDIZATION
PHYLOGENETIC ANALYSIS
BACTERIAL DIVERSITY
BOREAL FOREST
VICTORIA LAND
KINGDOM CRENARCHAEOTA
MICROBIAL COMMUNITIES
ARCHAEAL ASSEMBLAGES
PLANT-ROOTS
ABUNDANCE
Antarctic
Ross Sea
Victoria Land
Antarc*
Antarctica
Online Access:http://hdl.handle.net/2292/7916
https://doi.org/10.1111/j.1462-2920.2009.02111.x
Description
Summary:The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from four locations. A total of 1452 clones were analysed by restriction fragment length polymorphism and assigned to 43 operational taxonomic units from which representatives were sequenced. Archaea were primarily restricted to coastal mineral soils which showed a predominance of Crenarchaeota belonging to group 1.1b (> 99% of clones). These clones were assigned to six clusters (A through F), based on shared identity to sequences in the GenBank database. Ordination indicated that soil chemistry and water content determined archaeal community structure. This is the first comprehensive study of the archaeal community in Antarctic soils and as such provides a reference point for further investigation of microbial function in this environment.

Similar Items