Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas

Acetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study...

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Bibliographic Details
Published in:Ecotoxicology and Environmental Safety
Main Authors: Bebianno, Maria João, Mello, A. C. P., Serrano, M. A. S., Flores-Nunes, F., Mattos, J. J., Zacchi, F. L., Piazza, C. E., Siebert, M. N., Piazza, R. S., Gomes, C. H. A. M., Melo, C. M. R., Bainy, A. C. D.
Format: Article in Journal/Newspaper
Language:English
Published: Academic Press Inc Elsevier Science 2017
Subjects:
Antiinflammatory drugs nsaids
Environmental risk-assessment
Clam ruditapes-philippinarum
Waste-water
Mytilus-galloprovincialis
Pharmaceutical compounds
Dreissena-polymorpha
Corbicula-fluminea
Biomarker approach
Marine organisms
Crassostrea gigas
Online Access:http://hdl.handle.net/10400.1/12985
https://doi.org/10.1016/j.ecoenv.2017.06.034
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Summary:Acetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study was to evaluate if similar effects were observed in oysters Crassostrea gigas, given their economic and ecological importance and world-wide distribution. Oysters were exposed for 1, 4 and 7 days to two different sublethal PAR concentrations (0, 1 and 100 mu g L-1). Cell viability, DNA damage in hemocytes and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and glutathione S-transferases (GST) were evaluated in oyster gills. In addition, changes at transcriptional level of Cu/Zn superoxide distnutase (SOD), catalase-like (CAT-like), cytochrome P450 genes (CYP30C1, CYP2AU2, CYP3071A1, CYP356A1), glutathione S-transferase isoforms (GST-omega and GST-pi-like), cyclooxygenase (COX), fatty acid binding proteins-like (FABP-like), and caspase genes were evaluated in oyster gills and digestive gland. No changes in cell viability and DNA damage were observed in oysters exposed to both PAR concentrations. Similarly, no significant changes were detected in the major antioxidant enzymes (except for auxiliary enzyme GR) in oyster gills, suggesting that changes in GR activity are enough to counteract a potential oxidative stress in C. gigas gills under these experimental conditions. Furthermore, changes at transcriptional level are concentration and tissue dependent. PAR elicited an inhibition of CYP30C1, CYP3071A1 and FABP-like transcripts highlighting their role in drug metabolism, transport and detoxification of PAR in the gills. GST transcript levels were type, tissue and concentration-dependent. GST-pi-like was down-regulated in oyster gills exposed to the lowest PAR concentration and up-regulated in the ...

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