Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from crassostrea gigas nacre using a proteomic approach

Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimenta...

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Bibliographic Details
Published in:The Scientific World Journal
Main Authors: Oliveira, Daniel, Silva, Tome S., Cordeiro, Odete D., Cavaco, Sofia I., Simes, Dina
Format: Article in Journal/Newspaper
Language:English
Published: Hindawi Publishing Corporation 2012
Subjects:
Oyster pinctada-fucata
Tenascin-C
Organic matrix
Cathepsin-K
Cysteine proteinases
Bone-resorption
Cystatin-C
In-Vivo
Pacific oyster
Stefin-A
Pacific
Crassostrea gigas
Online Access:http://hdl.handle.net/10400.1/12065
https://doi.org/10.1100/2012/765909
Description
Summary:Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications. Portuguese Science and Technology Foundation (FCT) [SFRH/BD/41392/2007]

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