| Summary: | Dissertation (Ph.D.) University of Alaska Fairbanks, 2002 The genetic diversity at the Major Histocompatibility Complex (MHC) class II loci in some arctic mammalian species, musk ox, moose, caribou, and bears, have been characterized. The general objective of this study was to broaden the knowledge of the MHC polymorphism, selection, evolution and function in natural populations of arctic mammals. Allelic variation was assessed by analysis of MHC class II DR and DQ loci at exon 2 region. Sequences were amplified via the polymerase chain reaction (PCR), followed by either DNA sequencing after cloning of the PCR products or single-stranded conformation polymorphism (SSCP) analysis and sequencing. Monomorphism was observed at DRA, DRB, and DQA loci in both musk ox and moose, but relatively high polymorphism was observed at DQB locus. For the first time, four DQB alleles and one DQA allele were found identical in these two distantly related species which split approximately 23 million years ago, indicating stringent trans-species polymorphism. Both DRB and DQB seem to be functional by analyzing their cDNA expression. An intermediate level of MHC polymorphism at DRB locus was found in caribou and reindeer. Phylogenetic analysis of cervid DRB alleles indicated that all reindeer and caribou DRB alleles were from a monophyletic lineage, implying an ancient bottleneck in R. tarandus. High polymorphism at the DRB locus in polar bear was also observed. Four DRB alleles were found to be shared by polar bear and dog. The trans-species polymorphism of the shared alleles may have been persistent for 10 to 15 million years. Nine DQB alleles rather than two DRB alleles were also found in a pure domestic dog lineage of Doberman pinschers. These data imply that selection pressure may vary among MHC loci. In summary, the general level of MHC polymorphism at the class II loci is lower in herbivores (musk ox, moose, and caribou) than carnivores (polar bear). Biased selection may be applied on DQB locus. Stringent trans-species ...
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