Molecular characterization of the Rocky Mountain elk (Cervus elaphus nelsoni) PRNP putative promoter

© 2008 American Genetic Association Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD...

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Bibliographic Details
Published in:Journal of Heredity
Main Authors: Seabury, C., Gill, C., Templeton, J., Dyar, J., Derr, J., Adelson, D., Owens, E., Davis, D., Kraemer, D., Womack, J.
Format: Article in Journal/Newspaper
Language:English
Published: Oxford Univ Press Inc 2007
Subjects:
Animals
Deer
Wasting Disease
Chronic
Prions
DNA
Sequence Analysis
Base Sequence
Gene Frequency
Polymorphism
Single Nucleotide
Alleles
Colorado
Female
Male
Promoter Regions
Genetic
Alces alces
Online Access:http://hdl.handle.net/2440/46938
https://doi.org/10.1093/jhered/esm091
Description
Summary:© 2008 American Genetic Association Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) affecting deer (Odocoileus spp.), moose (Alces alces), and Rocky Mountain elk (Cervus elaphus nelsoni). Leucine homozygosity at elk PRNP codon 132 has been associated with reduced CWD susceptibility. However, naturally acquired CWD has been detected in elk possessing the 132 Leu/Leu genotype. Recent human and bovine studies indicate that PRNP regulatory polymorphisms may also influence TSE occurrence. Therefore, we generated sequences for the elk PRNP putative promoter (2.2 kb), exon 1 (predicted; 54 bp), intron 1 (predicted; 193 bp), and exon 3 (771 bp). Promoter prediction analysis using CpGProD yielded a single elk PRNP promoter that was homologous to regions of known promoter activity in cow and sheep. Molecular interrogation of the elk PRNP putative promoter revealed 32 diallelic single-nucleotide polymorphisms (SNPs). No variation was detected within the predicted exon 1 or intron 1 sequences. Evaluation of elk PRNP exon 3 revealed 3 SNPs (63Y, 312R, 394WMet/Leu). Bayesian haplotype reconstruction resulted in 3 elk PRNP haplotypes, with complete linkage disequilibrium observed between all PRNP putative promoter SNPs and codon 132. The results of this study provide the initial genomic foundation for future comparative and haplotype-based elk PRNP studies.

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