A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss

• Published in: Fungal Biology
• Main Authors: Minor, Kirsty L., Anderson, Victoria L., Davis, Katie S., Van Den Berg, Albert H., Christie, James S., Loebach, Lars, Faruk, Ali Reza, Wawra, Stephan, Secombes, Chris J., Van West, Pieter
• Other Authors: University of Aberdeen.Medical Sciences, University of Aberdeen.Scottish Fish Immunology Research Centre, University of Aberdeen.Rowett Institute, University of Aberdeen.Biological Sciences
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2014
• Subjects: SDG 3 - Good Health and Well-being; Oncorhynchus mykiss; Pathogenicity factor; Saprolegnia parasitica; Serine protease; salmo-trutta; brown trout; Atlantic salmon; malachite green; gene-expression; fish; pathogenicity; mortality; database; water; QH301 Biology; Biotechnology and Biological Sciences Research Council (BBSRC); QH301
• Online Access: http://hdl.handle.net/2164/5138; https://doi.org/10.1016/j.funbio.2014.04.008

Acknowledgements Our work was supported by the BBSRC (BB/C518457/1, BB/G012075/1, BB/J018333/1) (K.L.M., C.J.S., J.S.C., K.S.D., and P.v.W.), the University of Aberdeen (V.L.A., C.J.S., and P.v.W.), MSD Animal Health (J.S.C., K.S.D., and A.H.v.d.B), and The Royal Society (P.v.W.). This work was also supported by a Marie Curie Initial Training Networks with the SAPRO (sustainable approaches to reduce Oomycete (Saprolegnia) infections in aquacultures) grant PITN-GA-2009-238550 (A.H.v.d.B., L.L., C.J.S., P.v.W.). We would like to acknowledge Aberdeen Proteomics for carrying out LC–MS/MS and Laura Grenville-Briggs for valuable discussion and technical help. We are grateful to the Broad Institute (Carsten Russ, Rays Jiang, Brian Haas, and Chad Nusbaum), Brett Tyler (VBI), and P.v.W. for early release of draft supercontigs of the genome sequence of isolate CBS233.65, which helped us identify SpSsp1. Peer reviewed