Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions
The aim of this study was to optimize the content of EPA and DHA in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in structured triacylglycerols (TAGs). For this purpose, nonspecific lipase B from Candida antarctica immobilized...
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ftunintrujillojs:oai:ojs.revistas.unitru.edu.pe:article/1040 2023-05-15T13:43:45+02:00 Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions Maximización del contenido de Omega-3 (EPA y DHA) en el proceso de acidólisis enzimática de aceite de canola y concentrado de ácidos grasos poliinsaturados de cadena larga (AGPICL), en condiciones de CO2 supercrítico Cedano, José Rodríguez, Alicia Siche, Raúl 2016-12-05 application/pdf http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040 eng eng Universidad Nacional de Trujillo http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040/967 http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040/1312 http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040 Derechos de autor 2015 Scientia Agropecuaria Scientia Agropecuaria; Vol. 6 No. 4 (2015): October - December; 313-323 Scientia Agropecuaria; Vol. 6 Núm. 4 (2015): Octubre - Diciembre; 313-323 2306-6741 2077-9917 Optimization supercritical carbon dioxide structured triacylglycerols docosahexaenoic acid (DHA) eicosapentaenoico acid (EPA) Optimización dióxido de carbono supercrítico triacilglicéridos estructurados ácido eicosapentaenoico (EPA) ácido docosahexaenoico (DHA) info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftunintrujillojs 2022-06-04T18:43:11Z The aim of this study was to optimize the content of EPA and DHA in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in structured triacylglycerols (TAGs). For this purpose, nonspecific lipase B from Candida antarctica immobilized in a supercritical CO2 was used. Crude salmon oil obtained from the industrial byproducts was used to obtain LCPUFA concentrate. Initially, a LCPUFAs concentrate was obtained by basic hydrolysis and posterior complexation with urea. Subsequently the process variables were optimized enzymatic acidolysis were optimized using a central composite rotational design 25-1 + star, with 5 factors and 30 experimental trials, based on the response surface methodology. The optimal conditions that maximized the content of EPA and DHA to 3.92 g/100 g TFA and 9.09 g/100 g TFA, respectively in the purified TAGs corresponded to a LCPUFA percentage 71.71% and canola oil percentage 28.29%, temperature 57.8 °C, pressure 172.0 bar, time 23.97 h enzyme percentage of 7.74%. El objetivo del presente trabajo fue optimizar el contenido de ácidos grasos EPA y DHA en el proceso de acidólisis enzimática de aceite de canola y concentrado de ácidos grasos poliinsaturados de cadena larga (AGPICL) en triacilglicéridos estructurados (TAGs). Para ello, se empleó lipasa B inespecífica de Candida antarctica inmovilizada en condiciones CO2 supercrítico. El aceite crudo de salmón obtenido a partir de los subproductos industriales se utilizó para obtener concentrados de AGPICL. Como primer paso, se obtuvo un concentrado de AGPICL mediante una hidrolisis básica y posterior complejación con urea. Posteriormente se optimizó las variables del proceso de acidólisis enzimática mediante un diseño compuesto central rotacional 25-1 más estrella, de 5 factores con 30 ensayos experimentales, basado en la metodología superficie respuesta. Las condiciones óptimas que maximizaron el contenido de EPA a 3,92 g/100 g de ácidos grasos totales (AGT) y de DHA a 9,09 g/100 g ... Article in Journal/Newspaper Antarc* Antarctica Universidad Nacional de Trujillo: Publicaciones Científicas Cadena ENVELOPE(-67.600,-67.600,-67.450,-67.450) Larga ENVELOPE(-60.767,-60.767,-62.467,-62.467) Scientia agropecuaria 313 323 |
institution |
Open Polar |
collection |
Universidad Nacional de Trujillo: Publicaciones Científicas |
op_collection_id |
ftunintrujillojs |
language |
English |
topic |
Optimization supercritical carbon dioxide structured triacylglycerols docosahexaenoic acid (DHA) eicosapentaenoico acid (EPA) Optimización dióxido de carbono supercrítico triacilglicéridos estructurados ácido eicosapentaenoico (EPA) ácido docosahexaenoico (DHA) |
spellingShingle |
Optimization supercritical carbon dioxide structured triacylglycerols docosahexaenoic acid (DHA) eicosapentaenoico acid (EPA) Optimización dióxido de carbono supercrítico triacilglicéridos estructurados ácido eicosapentaenoico (EPA) ácido docosahexaenoico (DHA) Cedano, José Rodríguez, Alicia Siche, Raúl Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
topic_facet |
Optimization supercritical carbon dioxide structured triacylglycerols docosahexaenoic acid (DHA) eicosapentaenoico acid (EPA) Optimización dióxido de carbono supercrítico triacilglicéridos estructurados ácido eicosapentaenoico (EPA) ácido docosahexaenoico (DHA) |
description |
The aim of this study was to optimize the content of EPA and DHA in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in structured triacylglycerols (TAGs). For this purpose, nonspecific lipase B from Candida antarctica immobilized in a supercritical CO2 was used. Crude salmon oil obtained from the industrial byproducts was used to obtain LCPUFA concentrate. Initially, a LCPUFAs concentrate was obtained by basic hydrolysis and posterior complexation with urea. Subsequently the process variables were optimized enzymatic acidolysis were optimized using a central composite rotational design 25-1 + star, with 5 factors and 30 experimental trials, based on the response surface methodology. The optimal conditions that maximized the content of EPA and DHA to 3.92 g/100 g TFA and 9.09 g/100 g TFA, respectively in the purified TAGs corresponded to a LCPUFA percentage 71.71% and canola oil percentage 28.29%, temperature 57.8 °C, pressure 172.0 bar, time 23.97 h enzyme percentage of 7.74%. El objetivo del presente trabajo fue optimizar el contenido de ácidos grasos EPA y DHA en el proceso de acidólisis enzimática de aceite de canola y concentrado de ácidos grasos poliinsaturados de cadena larga (AGPICL) en triacilglicéridos estructurados (TAGs). Para ello, se empleó lipasa B inespecífica de Candida antarctica inmovilizada en condiciones CO2 supercrítico. El aceite crudo de salmón obtenido a partir de los subproductos industriales se utilizó para obtener concentrados de AGPICL. Como primer paso, se obtuvo un concentrado de AGPICL mediante una hidrolisis básica y posterior complejación con urea. Posteriormente se optimizó las variables del proceso de acidólisis enzimática mediante un diseño compuesto central rotacional 25-1 más estrella, de 5 factores con 30 ensayos experimentales, basado en la metodología superficie respuesta. Las condiciones óptimas que maximizaron el contenido de EPA a 3,92 g/100 g de ácidos grasos totales (AGT) y de DHA a 9,09 g/100 g ... |
format |
Article in Journal/Newspaper |
author |
Cedano, José Rodríguez, Alicia Siche, Raúl |
author_facet |
Cedano, José Rodríguez, Alicia Siche, Raúl |
author_sort |
Cedano, José |
title |
Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
title_short |
Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
title_full |
Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
title_fullStr |
Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
title_full_unstemmed |
Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditions |
title_sort |
maximizing content of omega-3 (epa and dha) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (lcpufa) in supercritical co2 conditions |
publisher |
Universidad Nacional de Trujillo |
publishDate |
2016 |
url |
http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040 |
long_lat |
ENVELOPE(-67.600,-67.600,-67.450,-67.450) ENVELOPE(-60.767,-60.767,-62.467,-62.467) |
geographic |
Cadena Larga |
geographic_facet |
Cadena Larga |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Scientia Agropecuaria; Vol. 6 No. 4 (2015): October - December; 313-323 Scientia Agropecuaria; Vol. 6 Núm. 4 (2015): Octubre - Diciembre; 313-323 2306-6741 2077-9917 |
op_relation |
http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040/967 http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040/1312 http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/1040 |
op_rights |
Derechos de autor 2015 Scientia Agropecuaria |
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Scientia agropecuaria |
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313 |
op_container_end_page |
323 |
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1766192654639431680 |