Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

• Published in: Environmental Science and Pollution Research
• Main Authors: Akcha, F., Barranger, A., Bachère, Evelyne, Heude Berthelin, C., Piquemal, D., Alonso, P., Rondon Sallan, R., Dimastrogiovanni, G., Porte, C., Menard, D., Szczybelski, A., Benabdelmouna, A, Auffret, Michel, Rouxel, J., Burgeot, T.
• Other Authors: Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), ACOBIOM, Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC), Centro de Investigaciones Biológicas (CSIC), Unité Amélioration génétique, Santé animale et Environnement (AGSAE), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ANR-11-CESA-0016,GIMEPEC,Génotoxicité, IMmunotoxicité et rEprotoxicité des Pesticides chez Crassostrea gigas(2011)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2016
• Subjects: Reproduction; Oyster; Pesticide; RT-qPCR; Gene expression; Histology; Immunity; Steroid; ACL; [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.science/hal-01363931; https://hal.science/hal-01363931/document; https://hal.science/hal-01363931/file/Akcha-2016-EnvSciPollutRes-Effects-MANUSCRIT.pdf; https://doi.org/10.1007/s11356-015-5969-2

International audience Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA