Study of electrical swing ion exchange : application of coupling of ion exchange-electrodialysis to biomolecules separation

• Main Author: Lu, Wei
• Other Authors: Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut National Polytechnique de Lorraine, Laurence Muhr, Georges Grevillot
• Format: Doctoral or Postdoctoral Thesis
• Language: French
• Published: HAL CCSD 2010
• Subjects: Ion exchange; Bipolar membrane; Amino acid; Water splitting; Electrodialysis; Green eluent; Peptide; Echange d'ions; Acide aminé; Membrane bipolaire; Dissociation de l'eau; Eluant propre; Electrodialyse; Peptides; Acides aminés; [SPI.OTHER]Engineering Sciences [physics]/Other; [PHYS.COND.CM-GEN]Physics [physics]/Condensed Matter [cond-mat]/Other [cond-mat.other]; Carbonic acid
• Online Access: https://hal.univ-lorraine.fr/tel-01748797; https://hal.univ-lorraine.fr/tel-01748797/document; https://hal.univ-lorraine.fr/tel-01748797/file/2010_LU_W.pdf

The present work aims to study the coupling of ion exchange and electrodialysis. This study is applied to the separation of biomolecules. One objective is to reduce the generation of saline wastewater produced by the ion exchange steps used conventionally in bioseparations. One approach has led to the design of architecture with a cyclic mode in 3 steps to purify some families of peptides without using a buffer pH or generate wastes. The experimental device consists of an electrodialysis cell in which are introduced anion exchange resins. The three steps are: loading of biomolecules on the resin initially in the carbonate form, elution with a solution of carbon dioxide dissolved in water, electroregeneration of the resin in its original form leading simultaneously to the regeneration of the carbonic acid solution. Using a modelling of the electroregeneration step, simulations can improve the understanding of coupled processes as the ion exchange equilibria, the equilibria in solution, the electromigration. A second approach has then been to study the possibilities of controlling the pH by electrochemical means to limit the use of buffers. The dissociation of water, leading to the formation of protons and hydroxyl ions, has been particularly studied by accounting the properties of contacts called « bipolar » as a result of an electric field. It was established that the choice of resin type and the current density can modify the pH. However this work must be pursued through research of architectures and operating procedures that deliver appropriate buffer capacity Le présent travail vise à étudier le couplage de l'échange d'ions et de l'électrodialyse. Cette étude est appliquée à la séparation de biomolécules. Un des objectifs est de diminuer la génération d'effluents salins produits par les étapes d'échange d'ions utilisées de façon classique dans les bioséparations. Une première approche a conduit à la conception d'une architecture avec un mode de fonctionnement cyclique en 3 étapes qui permet de purifier ...